Dietary fructose causes defective insulin signalling and ceramide accumulation in the liver that can be reversed by gut microbiota modulation

  • Raffaella Crescenzo
  • Arianna Mazzoli
  • Blanda Di Luccia
  • Francesca Bianco
  • Rosa Cancelliere
  • Luisa Cigliano
  • Giovanna Liverini
  • Loredana Baccigalupi
  • Susanna Iossa
Keywords: Fructose, insulin, obesity, inflammation, microbiota

Abstract

Objective : The link between metabolic derangement of the gut–2013liver–visceral white adipose tissue (v-WAT) axis and gut microbiota was investigated.

Methods : Rats were fed a fructose-rich diet and treated with an antibiotic mix. Inflammation was measured in portal plasma, ileum, liver, and v-WAT, while insulin signalling was analysed by measuring levels of phosphorylated kinase Akt. The function and oxidative status of hepatic mitochondria and caecal microbiota composition were also evaluated.

Results : Ileal inflammation, increase in plasma transaminases, plasma peroxidised lipids, portal concentrations of tumour necrosis factor alpha, lipopolysaccharide, and non-esterified fatty acids, were induced by fructose and were reversed by antibiotic. The increased hepatic ceramide content, inflammation and decreased insulin signaling in liver and v-WAT induced by fructose was reversed by antibiotic. Antibiotic also blunted the increase in hepatic mitochondrial efficiency and oxidative damage of rats fed fructose-rich diet. Three genera, Coprococcus, Ruminococcus, and Clostridium, significantly increased, while the Clostridiaceae family significantly decreased in rats fed a fructose-rich diet, and antibiotic abolished these variations

Conclusions : When gut microbiota modulation by fructose is prevented by antibiotic, inflammatory flow from the gut to the liver and v-WAT are reversed.

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Published
2017-06-09
How to Cite
1.
Crescenzo R, Mazzoli A, Di Luccia B, Bianco F, Cancelliere R, Cigliano L, Liverini G, Baccigalupi L, Iossa S. Dietary fructose causes defective insulin signalling and ceramide accumulation in the liver that can be reversed by gut microbiota modulation. Food & Nutrition Research [Internet]. 9Jun.2017 [cited 22Apr.2018];61. Available from: http://foodandnutritionresearch.net/index.php/fnr/article/view/1205
Section
Original Articles