@article{Kim_Kim_Kim_Choi_2017, title={Anti-adipogenic effects of <em>Tropaeolum majus</em> (nasturtium) ethanol extract on 3T3-L1 cells}, volume={61}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/1237}, abstractNote={<p>Background : Edible flowers, Tropaeolum majus has been used as a disinfectant and an antibiotic, and for wound healing, but the anti-obesity effects of this plant have not been reported previously</p> <p>Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells.</p> <p>Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid accumulation levels were determined using Oil-Red O staining and a triglyceride content assay. Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting.</p> <p>Results : The highest inhibition of lipid accumulation was observed at a TME concentration of 300 µg/mL. Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. This decrease was shown to be concentration-dependent.</p> <p>Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells.</p> <p>Conclusions : TME may be a potential novel therapeutic agent for the prevention and treatment of obesity.</p&gt;}, journal={Food & Nutrition Research}, author={Kim Gi-Chang and Kim Jin-Sook and Kim Gyoung-Mi and Choi Song-Yi}, year={2017}, month={Jun.} }