@article{Khan_Khan_Sahreen_Alkreathy_2016, title={Effect of <em>Launaea procumbens</em> extract on oxidative marker, p53, and CYP 2E1: a randomized control study}, volume={60}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/1037}, DOI={10.3402/fnr.v60.29790}, abstractNote={<p><em><strong>Background</strong></em>: Ethyl acetate extracts of <em>Launaea procumbens</em> is used for the treatment of liver dysfunction as an herbal medicine in Pakistan. In this study, the protective effects of ethyl acetate extracts were evaluated against CCl<sub>4</sub>-induced liver injuries in rat.</p><p><em><strong>Methods</strong></em>: To examine the protective effects against oxidative stress of carbon tetrachloride in rats, 30 male rats were equally divided into 5 groups (6 rats). Among five groups, one was treated with CCl<sub>4</sub> (3 ml/kg i.p. in olive oil b.w.) twice a week for 4 weeks. Others were orally fed with extracts (100, 200 mg/kg b.w.), with CCl<sub>4</sub> twice a week for 4 weeks.</p><p><em><strong>Results</strong></em>: Administration of CCl<sub>4</sub> altered the serum marker enzymes, lipid profile, CYP 2E1, p53 expression, antioxidant enzymes, nuclear organizer regions (AgNORs), and DNA. Supplement of <em>L. procumbens</em> ameliorated the effects of CCl<sub>4</sub>, improved CYP 2E1, p53, and increased the activities of antioxidant enzymes while activity of liver marker enzymes (ALP, ALT, AST, g-GT) and contents of lipid per oxidation contents (TBARS), AgNORs, and DNA fragmentation were decreased. Similarly body weight was increased while liver and relative liver weight was decreased with co-administration of various extracts, suggesting that <em>L. procumbens</em> effectively protect liver against the CCl<sub>4</sub>-induced oxidative damage in rats.</p><p><em><strong>Conclusion</strong></em>: The hepatoprotective and free radical scavenging effects might be due to the presence of bioactive constituents in the extract.</p><p>Keywords: <em>carbon tetrachloride; Launaea procumbens; liver; hepatic antioxidants; CYP 2E1; lipid peroxidation; p53; AgNORs</em></p><p>(Published: 3 March 2016)</p><p>Citation: Food &amp; Nutrition Research 2016, 60: 29790 - http://dx.doi.org/10.3402/fnr.v60.29790</p&gt;}, journal={Food & Nutrition Research}, author={Khan Rahmat Ali and Khan Muhammad Rashid and Sahreen Sumaira and Alkreathy Huda Mohammad}, year={2016}, month={Mar.} }