@article{Potenza_Montagnani_Nacci_De Salvia_2016, title={Chronic treatment with epigallocatechin gallate reduces motor hyperactivity and affects<em>in</em> vitro tested intestinal motility of spontaneously hypertensive rats}, volume={60}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/1042}, DOI={10.3402/fnr.v60.28373}, abstractNote={<p><em><strong>Background</strong></em>: Green tea catechins seem to contribute toward reducing body weight and fat.</p><p><em><strong>Objective</strong></em>: We aimed to investigate whether chronic administration of (–)-epigallocatechin-3-gallate (EGCG), the most abundant catechin of green tea, reduces weight gain in spontaneously hypertensive rats (SHR), an animal model of metabolic syndrome, by increasing motor activity and/or by altering gastrointestinal motility.</p><p><em><strong>Design</strong></em>: Nine-week-old SHR were randomly assigned to two groups and treated by gavage for 3 weeks with vehicle dimethyl sulfoxide or EGCG (200 mg/kg/day). Age-matched Wistar-Kyoto (WKY) control rats were treated with vehicle alone. The effect of chronic administration of EGCG was evaluated on open-field motor activity and on <em>ex vivo</em> colonic and duodenal motility. Moreover, <em>in vitro</em> acute effect of 20-min incubation with EGCG (100 µM) or vehicle was evaluated in colonic and duodenal specimens from untreated WKY rats and SHR.</p><p><em><strong>Results</strong></em>: Vehicle-treated SHR were normoglycemic and hyperinsulinemic, and showed a reduction of plasma adiponectin when compared to vehicle-treated WKY rats. In addition, consistent with fasting glucose and insulin values, vehicle-treated SHR were more insulin resistant than age-matched vehicle-treated WKY rats. Chronic treatment for 3 weeks with EGCG improved insulin sensitivity, raised plasma adiponectin levels, and reduced food intake and weight gain in SHR. Vehicle-treated SHR showed increased open-field motor activity (both crossings and rearings) when tested after each week of treatment. The overall hyperactivity of vehicle-treated SHR was significantly reduced to the levels of vehicle-treated WKY rats after 2 and 3 weeks of EGCG treatment. Colonic and duodenal preparations obtained from SHR chronically treated <em>in vivo</em> with EGCG showed reduced responses to carbachol (0.05–5 µM) and increased inhibitory response to electrical field stimulation (EFS, 1–10 Hz, 13 V, 1 msec, 10-sec train duration), respectively. <em>In vitro</em> acute EGCG incubation (100 µM, 20 min) of colonic and duodenum strips obtained from untreated SHR and WKY rats showed a reduced contractile colonic response to a fixed dose of carbachol (1.5 µM) only in SHR with respect to its own vehicle, whereas the inhibitory duodenal response to a fixed EFS frequency (5 Hz) was significantly reduced in both WKY rats and SHR groups with respect to their own vehicle.</p><p><em><strong>Conclusions</strong></em>: These data suggest that EGCG affects body weight gain in rats and this effect seems to be due to the altered intestinal motility and not to increased motor activity.</p><p>Keywords: <em>green tea; weight gain; colon; duodenum</em></p><p><em>(Published: 17 February 2016)</em></p><p><em>Citation: Food &amp; Nutrition Research 2016, 60: 28373 - http://dx.doi.org/10.3402/fnr.v60.28373</em></p&gt;}, journal={Food & Nutrition Research}, author={Potenza Maria Assunta and Montagnani Monica and Nacci Carmela and De Salvia Maria Antonietta}, year={2016}, month={Feb.} }