@article{Song_Wen_Sun_Bai_Jiao_Hu_Peng_He_Ou_2016, title={Cytoprotective mechanism of ferulic acid against high glucose-induced oxidative stress in cardiomyocytes and hepatocytes}, volume={60}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/1046}, DOI={10.3402/fnr.v60.30323}, abstractNote={<p><em><strong>Background</strong></em>: Ferulic acid (FA), a phenolic acid, is a potential therapy for diabetes mellitus. FA has been shown to protect against hepatic and myocardial injury and oxidative stress in obese rats with late-stage diabetes, but the mechanism of the antioxidative activity of FA is still unclear.</p><p><em><strong>Objective</strong></em>: The aim of this study was to elucidate whether FA can prevent damage to cardiomyocytes and hepatocytes caused by high glucose (HG)-induced oxidative stress and whether the protection effects of FA on these cells are related to the Keap1-Nrf2-ARE signaling pathways.</p><p><em><strong>Design</strong></em>: Cells were divided into four groups: a control group (cultured with normal medium), an HG group (medium containing 80 mmol/L glucose), an FA+HG group (medium containing 80 mmol/L glucose and 1, 5, or 10 µg/mL FA), and a dimethylbiguanide (DMBG)+HG group (medium containing 80 mmol/L glucose and 50 µg/mL DMBG).</p><p><em><strong>Results</strong></em>: FA treatment significantly increased cell viability and significantly decreased cell apoptosis compared with the HG-treated group. Moreover, FA down-regulated the expression of Keap1 protein and up-regulated the expression of Nrf2 protein and gene transcription of HO-1 and glutathione S-transferase (GST) in a dose-dependent manner.</p><p><em><strong>Conclusion</strong></em>: FA alleviated the HG-induced oxidative stress and decreased cell apoptosis in hepatocytes and cardiomyocytes. These effects were associated with the Keap1-Nrf2-ARE signaling pathway.</p><p>Keywords: <em>ferulic acid; oxidative stress; cardiomyocytes; hepatocytes</em></p><p>(Published: 10 February 2016)</p><p>Citation: Food &amp; Nutrition Research 2016, 60: 30323 - http://dx.doi.org/10.3402/fnr.v60.30323</p&gt;}, journal={Food & Nutrition Research}, author={Song Yuan and Wen Luona and Sun Jianxia and Bai Weibin and Jiao Rui and Hu Yunfeng and Peng Xichun and He Yong and Ou Shiyi}, year={2016}, month={Feb.} }