@article{Jing_Hu_Song_2020, title={Lycium barbarum polysaccharide (LBP) inhibits palmitic acid (PA)-induced MC3T3-E1 cell apoptosis by regulating miR-200b-3p/<em>Chrdl1</em>/PPARγ}, volume={64}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/4208}, DOI={10.29219/fnr.v64.4208}, abstractNote={<p><strong>Background</strong>: Obesity is closely related to osteoporosis. Lycium barbarum polysaccharides (LBPs) have anti- osteoporosis activity.</p> <p><strong>Objective</strong>: This study aimed to explore the role of LBPs in palmitic acid (PA)-induced osteoblast apoptosis.</p> <p><strong>Methods</strong>: The microarray data set <em>GSE37676</em> was downloaded from Gene Expression Ominibus (GEO) database. Top 300 differentially expressed genes (DEGs) were used to construct a protein–protein interaction (PPI) network based on STRING database, and significant modules were analyzed and their key genes were screened by using Cytoscape software. COEXPEDIA database showed that there was co-expression between Chrdl1 and peroxisome proliferator-activated receptor (PPARγ). MC3T3-E1 cells were treated with 100–500 μg/mL of PA. Reverse transcription polymerase chain reaction (RT-PCR) and western blot assays were used to detect mRNA and protein levels. Cell Counting Kit-8 (CCK-8) assay and flow cytometry were used to detect cell viability and cell apoptosis.</p> <p><strong>Results</strong>:<em> Chrdl1</em> was the key gene from the most significant module and downregulation in MC3T3-E1 cells treated with PA. MicroRNA miR-200b-3p and PPARγ were significantly upregulated among PA-treated MC3T3-E1 cells. The results of luciferase reporter gene assay showed that miR-200b-3p targeted <em>Chrdl1</em> 3’-UTR. Over-expressing miR-200b-3p promoted PA-induced cell apoptosis and inhibited cell viability. After pre-treating cells with PA and LBP, MC3T3-E1 cell apoptosis rate was relatively lower than that of mimics+PA200 group.<em> Chrdl1</em> inhibition partly reversed miR-200b-3p effect on inhibiting apoptosis among MC3T3-E1 cells pre-treated with LBP and PA. Decreased C CASP3, PPARγ and increased <em>Chrdl1</em> by miR- 200b-3p inhibition were partly reversed by <em>Chrdl1</em> inhibition.</p> <p><strong>Conclusions</strong>: LBPs inhibit PA-induced MC3T3-E1 cell apoptosis by mainly decreasing miR-200b-3p to upregulate Chrdl1, but miR-200b-3p/<em>Chrdl1</em>/PPARγ is not the only mechanism for LBPs protecting osteoblasts from PA.</p&gt;}, journal={Food & Nutrition Research}, author={Jing Lei and Hu Baiwen and Song Qing Hua}, year={2020}, month={Dec.} }