@article{Li_Lu_Huang_Han_Yang_2016, title={Effects of stachyose on absorption and transportation of tea catechins in mice: possible role of Phase II metabolic enzymes and efflux transporters inhibition by stachyose}, volume={60}, url={https://foodandnutritionresearch.net/index.php/fnr/article/view/971}, DOI={10.3402/fnr.v60.32783}, abstractNote={<p><em><strong>Background</strong></em>: Nutritional and absorption-promoting properties of stachyose combined with tea catechins (TC) have been revealed. However, the mechanism involved in non-digestible oligosaccharides-mediated enhancement of flavonoid absorption has largely remained elusive.</p><p><em><strong>Methods</strong></em>: This study was designed to investigate the molecular mechanism of stachyose in enhancing absorption and transportation of TC in mice. Mice were orally pre-treated with stachyose (50, 250, and 500 mg/kg·bw) for 0–8 weeks, and 1 h before sacrifice, mice were treated with TC (250 mg/kg·bw).</p><p><em><strong>Results</strong></em>: Gas chromatography-mass spectrometry analysis showed that serum concentrations of epicatechin, epigallocatechin, epicatechin gallate, and epigallocatechin gallate were dose- and time-dependently elevated with stachyose pre-treatment in mice. Furthermore, pre-treatment with stachyose in mice reduced intestinal sulfotransferase and uridine diphosphate-glucuronosyltransferase levels by 3.3–43.2% and 23.9–30.4%, relative to control mice, respectively. Moreover, intestinal P-glycoprotein and multidrug resistance-associated protein-1 contents were decreased in mice by pre-administration of stachyose in dose- and time-dependent manner.</p><p><em><strong>Conclusions</strong></em>: This is the first time to demonstrate that suppression of Phase II metabolic enzymes and efflux transporters of TC in the intestine can play a major role in increasing absorption of TC by stachyose feeding.</p><p>Keywords: <em>absorption; efflux transporter; Phase II metabolism; stachyose; tea catechins</em></p><p>(Published: 24 October 2016)</p><p>Citation: Food &amp; Nutrition Research 2016, 60: 32783 - http://dx.doi.org/10.3402/fnr.v60.32783</p&gt;}, journal={Food & Nutrition Research}, author={Li Wenfeng and Lu Yalong and Huang Di and Han Xiao and Yang Xingbin}, year={2016}, month={Oct.} }