TY - JOUR AU - You , Lang AU - Li , Fengxia AU - Sun , Yan AU - Luo , Liang AU - Qin , Jian AU - Wang , Tao AU - Liu , Yuchen AU - Lai , Ruogu AU - Li , Ruohan AU - Guo , Xiaoran AU - Mai , Qiuyan AU - Pan , Yihang AU - Xu , Jianrong AU - Li , Ningning PY - 2021/03/01 Y2 - 2024/03/29 TI - Extract of <em>Acalypha australis L. </em>inhibits lipid accumulation and ameliorates HFD-induced obesity in mice through regulating adipose differentiation by decreasing PPARγ and CEBP/α expression JF - Food & Nutrition Research JA - fnr VL - 65 IS - SE - Original Articles DO - 10.29219/fnr.v65.4246 UR - https://foodandnutritionresearch.net/index.php/fnr/article/view/4246 SP - AB - Background: Obesity is a principal risk factor for the development of type 2 diabetes and cardiovascular diseases. Natural plants and/or foods play an important role in the management of obesity. Acalypha australis L. (AAL) is a kind of potherb popular among Asian populations, and it is also consumed as a food ingredient and traditional herbal medicine.Objective: We investigated the effects of water extract from AAL on high-fat-diet (HFD)-induced obese mice and 3T3-L1 adipocytes to develop a new functional food material.Design: Nine-week-old male mice were randomly divided into control (chow diet, n = 6) and HFD (n = 30) group. From 12-weeks onward, mice in the HFD group were further separated into model (saline, 6 mL/ kg), simvastatin (0.11 mg/mL, 6 mL/kg), and AAL treatment (low, middle, and high dosage: 300, 600, and 900 mg/kg) group, with 6 animals per group, while mice in the control group were treated with saline (6 mL/ kg). Food intake, body/fat weight, liver/kidney indexes, and lipid profiles were determined. Tissues were fixed with formalin for pathological examination. Western blotting and PCR were performed to evaluate the protein and mRNA expression in 3T3-L1 adipocytes. Oil Red O staining was used to determine lipid accumulation.Results: AAL administration significantly suppressed body weight gain, and reduced fat pad weight and Lee’s index in obese mice, but had no effect on liver/kidney index. AAL also reduced serum cholesterol, triglyceride, and LDL-C and increased HDL-C levels. Histological analysis revealed that AAL significantly ameliorated lipid accumulation in the liver and subcutaneous adipose tissue. In vitro, Oil Red O staining showed that AAL inhibited adipose differentiation by down-regulating the gene and protein expression of PPARγ and C/EBPα. AAL also reversed HFD-induced intestinal dysbacteriosis.Conclusion: AAL water-soluble extract has a significant anti-adipogenic effect in the HFD-induced obese mice model. ER -