Anti-inflammatory effects of potato extract on a rat model of cigarette smoke - induced chronic obstructive pulmonary disease

  • Gui Hua Xu Department of Clinical Medical Research Center, Inner Mongolia Autonomous Region People's Hospital, Hohhot 010017, Inner Mongolia
  • Jie Shen Department of Neurology, Inner Mongolia Autonomous Region People's Hospital, Hohhot 010017, Inner Mongolia
  • Peng Sun Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Min Li Yang Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University
  • Peng Wei Zhao Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Yan Niu Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Jing Kun Lu Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Zhi Qiang Wang Department of Anatomy, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Chao Gao Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Xue Han Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Lei Lei Liu Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Chen Chen Liu Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
  • Zhang Yue Cong Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia
Keywords: Potato extract, Cigarette smoke, Chronic Obstructive Pulmonary Disease, Inflammation

Abstract

Objective: This study aimed to evaluate the therapeutic effects of potato extract (PE) on cigarette smoke (CS)–induced chronic obstructive pulmonary disease (COPD).

Methods: PE was first prepared by frozen centrifugation, and its amino acid composition was detected. Toxicity of PE was analyzed by changes in morphology, behavior, routine blood indexes, and biochemical criteria of mice. Then, the COPD rat model was established by CS exposure, and PE, doxofylline, and prednisolone acetate were used to treat these rats. After 45 days of treatment, the morphology and behavior of rats were recorded. In addition, the histopathology of lung tissue was evaluated by chest x-ray and hematoxylin and eosin staining. The expression of interleukine-10 (IL-10), tumor necrosis factor-α (TNF-α), and granulocyte colony-stimulating factor (G-CSF) was detected in serum and lung tissue by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively.

Results: Various amino acids were identified in PE, and no toxicity was exhibited in mice. The CS-induced COPD rat model was successfully established, which exhibited significant thickened and disordered lung markings on 90% of the rats. After administering doxofylline and prednisolone acetate, inflammation symptoms were improved. However, side effects such as emaciation, weakness, and loosening of teeth appeared. In the PE group, obviously improved histopathology was observed in lung tissues. Meanwhile, it was revealed that PE could increase the expression of IL-10 and reduce the expression of TNF-α and G-CSF in COPD rats, and doxofylline and prednisolone acetate also elicited similar results.

Conclusion: Our study suggests PE might be effective in the treatment of CS-induced COPD by inhibiting inflammation.

Keywords: potato extract; cigarette smoke; chronic obstructive pulmonary disease; inflammation

(Published: 23 October 2015)

Citation: Food & Nutrition Research 2015, 59: 28879 - http://dx.doi.org/10.3402/fnr.v59.28879

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Published
2015-10-23
How to Cite
Xu, G. H., Shen, J., Sun, P., Yang, M. L., Zhao, P. W., Niu, Y., Lu, J. K., Wang, Z. Q., Gao, C., Han, X., Liu, L. L., Liu, C. C., & Cong, Z. Y. (2015). Anti-inflammatory effects of potato extract on a rat model of cigarette smoke - induced chronic obstructive pulmonary disease. Food & Nutrition Research, 59. https://doi.org/10.3402/fnr.v59.28879
Section
Original Articles