Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis

  • Gabriel Betanzos-Cabrera Universidad Autónoma del Estado de Hidalgo
  • Perla Y. Montes-Rubio Universidad Autónoma del Estado de Hidalgo
  • Héctor E. Fabela-Illescas Universidad Autónoma del Estado de Hidalgo
  • Helen Miller-Belefant Dale Bumpers National Rice Research Center, Stuttgart, AR, USA
  • Juan C. Cancino-Diaz Escuela Nacional Ciencias Biológicas del Instituto Politécnico Nacional, México City
Keywords: antioxidant, antibacterial, polyphenols

Abstract

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray® had a MIC100% at 20%, followed by Del Valle® with a MIC15% at 20% concentration only. The beverages Jumex® and Sonrisa® did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

Keywords: pomegranate juice; Staphylococcus epidermidis; antimicrobial activity; antioxidant capacity; ocular infections

(Published: 20 May 2015)

Citation: Food & Nutrition Research 2015, 59: 27620 - http://dx.doi.org/10.3402/fnr.v59.27620

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Author Biographies

Gabriel Betanzos-Cabrera, Universidad Autónoma del Estado de Hidalgo
Área Académica de Nutrición
Perla Y. Montes-Rubio, Universidad Autónoma del Estado de Hidalgo
Área Académica de Nutrición
Héctor E. Fabela-Illescas, Universidad Autónoma del Estado de Hidalgo
Área Académica de Nutrición
Juan C. Cancino-Diaz, Escuela Nacional Ciencias Biológicas del Instituto Politécnico Nacional, México City

Departamento de Microbiología.

Published
2015-05-20
How to Cite
Betanzos-Cabrera, G., Montes-Rubio, P. Y., Fabela-Illescas, H. E., Miller-Belefant, H., & Cancino-Diaz, J. C. (2015). Antibacterial activity of fresh pomegranate juice against clinical strains of <em>Staphylococcus epidermidis</em&gt;. Food & Nutrition Research, 59. https://doi.org/10.3402/fnr.v59.27620
Section
Original Articles