Mechanisms of combined deer antler polysaccharides and postbiotics supplementation for regulating obesity in mice

Lanyue Yin; Jiating Li; Xueyue Tai; Guoqi Zhang; Mingran Luan; Bao Zhong; Fenglin Li

doi:10.29219/fnr.v69.11634

Lanyue Yin College of Food Science and Nutrition Engineering, Jilin Agricultural Science and Technology University, Jilin, China
Jiating Li School of Public health, Jilin Medical University, Jilin, P.R. China
Xueyue Tai College of Food Science and Engineering, Changchun University, Changchun, China
Guoqi Zhang College of Food Science and Nutrition Engineering, Jilin Agricultural Science and Technology University, Jilin, China; College of Food Science and Engineering, Changchun University, Changchun, China
Mingran Luan College of Food Science and Nutrition Engineering, Jilin Agricultural Science and Technology University, Jilin, China
Bao Zhong College of Food Science and Nutrition Engineering, Jilin Agricultural Science and Technology University, Jilin, China
Fenglin Li College of Food Science and Nutrition Engineering, Jilin Agricultural Science and Technology University, Jilin, China

DOI: https://doi.org/10.29219/fnr.v69.11634

Keywords: deer antler polysaccharides, postbiotics, lipid metabolism, regulatory effects

Abstract

Objective: This study investigated the mechanisms related to lipid metabolism regulation after combined supplementation with deer antler polysaccharides and postbiotics.

Methods: Thirty-two male mice were divided into high-fat diet, HD + deer antler polysaccharides, HD + Bacillus coagulans postbiotics, and HD + deer antler polysaccharides + B. coagulans postbiotics groups. The diets contained 60% fat. After 9 weeks, the effects of deer antler polysaccharides and postbiotics on lipid metabolism were assessed through blood biochemical, histological tissue staining, and polymerase chain reaction analyses.

Results: Supplementation with deer antler polysaccharides and postbiotics significantly inhibited weight gain in obese mice, reduced serum total cholesterol, triglyceride, and low-density lipoprotein levels and markedly increased the serum high-density lipoprotein level. Additionally, hepatic lipid droplet accumulation and adipocyte hypertrophy improved. The expressions of the lipid synthesis genes, sterol regulatory element-binding protein 1 (i.e. SREBP-1c), and fatty acid synthase (i.e. FAS), significantly decreased, while peroxisome proliferator-activated receptor alpha (i.e. PPAR-α) and acyl-CoA oxidase 1 (i.e. ACOX1) expression significantly increased. The expressions of the inflammation-related genes, tumor necrosis factor-alpha (i.e. TNF-α), interleukin (IL)-6, and IL-1 also significantly decreased.

Conclusion: Thus, combined deer antler polysaccharides and postbiotic supplementation regulated obesity in mice, potentially by modulating lipid synthesis and inflammation-related gene expression.

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