Allium hookeri root extract restores streptozotocin-induced pancreatic β-cells dysfunction in a type 1 diabetic rat model

Hyun Ju Kim; Seong-Soo Roh; Sung-Hyen Lee; Miran Kang; Jong-Sik Jin

doi:10.29219/fnr.v69.12104

Hyun Ju Kim Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju, Republic of Korea
Seong-Soo Roh ollege of Korean Medicine, Daegue Haany University, Daegu, Republic of Korea
Sung-Hyen Lee Functional Food Division, Department of Agro-food resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Jeonbuk, Republic of Korea
Miran Kang Kimchi Industry Promotion Division, World Institute of Kimchi, Gwangju, Republic of Korea
Jong-Sik Jin Department of Pharmacy, Jeonbuk National University, Iksan, Republic of Korea;; DLED-Agri-bio Fusion Technology Research Center, Jeonbuk National University, Iksan, Republic of Korea

DOI: https://doi.org/10.29219/fnr.v69.12104

Keywords: Allium hookeri root, diabetes, oxidative stress, apoptosis, β-cell regeneration

Abstract

Background: Allium hookeri (AH), a traditional herb in Southeast Asia, has been documented for its significant health benefits in metabolic diseases. This study was to explore the effects of AH root extract (AHRE) on pancreatic β-cell regeneration in streptozotocin (STZ)-induced diabetic rats.

Methods: AHRE (100 mg/kg body weight) was administered daily to STZ-induced diabetic rats for 2 weeks. Serum glucose and insulin levels, total-cholesterol, hemoglobin A1c, alanine transaminase, aspartate transaminase, and pancreatic peroxynitrite and thiobarbituric acid reactive substances were measured. Protein expression associated with pancreatic β-cell apoptosis and regeneration was analyzed through Western blotting.

Results: Diabetic rats exhibited hyperglycemia, insulin deficiency, increased levels of oxidative stress markers, and pancreatic β-cell apoptosis and impairment. AHRE treatment reduced hyperglycemia, insulin insufficiency, and oxidative stress, implying a reduction in pancreatic β-cell apoptosis and restoration of pancreatic β-cell regeneration-associated protein expression.

Conclusions: AHRE can facilitate β-cell regeneration in the impaired pancreatic islets through STZ by reducing oxidative stress markers and apoptosis in pancreatic tissue. Owing to pancreatic β-cells are susceptible to oxidative stress, the protective and enhancing effects of AHRE on the apoptosis and regeneration of these cells may be a significant mechanism for its hypoglycemic effect.

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