Rhus verniciflua leaf extract suppresses obesity in high-fat diet-induced obese mice
Background: Obesity is a serious health problem in the world. We thought that the activity and safety of natural plants and/or foods are very important in the management of therapy for obesity. Rhus verniciflua (R. verniciflua) is also known as lacquer tree in Japan and Korea, and it is consumed as food ingredients and/ or traditional herbal medicine. We prepared an extract from R. verniciflua leaves (Rv-PEM01-99) to develop a new functional food material and/or nutritional supplements.
Objective: This study evaluated the anti-obesity effects of the Rv-PEM01-99 in high-fat diet (HFD)-induced obese mice.
Design: Six-week-old male C57BL/6J mice were divided into three groups: group I (HFD group), group II (HFD + 1% Rv-PEM01-99 group), and group III (HFD + 2% Rv-PEM01-99 group). Throughout the 56-day treatment period, body weights of these mice were checked twice a week. After 56 days, blood biochemical analyses were performed.
Results: In animal studies, no death or abnormalities in food consumption were observed between groups I, II, and III. Body weight gain in the groups administered Rv-PEM01-99 was less than that in group I. In particular, body weight gain in group III was significantly less than that in group I after 52 days of Rv-PEM01-99 administration. In addition, intra-abdominal fat and leptin levels in group III were significantly lower than those in group I. HPLC and LC/MS analysis showed a quercetin derivative as an active compound in Rv-PEM01-99.
Conclusion: Rv-PEM01-99, containing a quercetin derivative, showed anti-obesity effect in HFD-fed mice. It could therefore be useful as food material and/or nutritional supplement for management of obesity.
- Finucane MM, Stevens GA, Cowan MJ, Danaei G, Lin PK, Paciorek CJ, et al. National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9.1 million participants. Lancet 2011; 377: 557–67. doi: 10.1016/S0140-6736(10)62037-5
- World Health Organization [WHO] and Global Health Observatory [GHO]. Overweight and obesity [updated 2017]. Available from: http://www.who.int/gho/ncd/risk_factors/overweight/en/
- World Health Organization [WHO]. 10 Facts on obesity. [updated October 2017]. Available from: http://www.who.int/features/factfiles/obesity/zh/index.html
- Poirier P, Giles TD, Bra GA, Hong Y, Stern JS, Pi-Sunyer FX, et al. Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss. Arterioscler Thromb Vasc Biol 2006; 26: 968–76. doi: 10.1161/01.ATV.0000216787.85457.f3
- Haslam DW, James WP. Obesity. Lancet 2005; 366: 1197–209. doi: 10.1016/S0140-6736(05)67483-1
- Maguire T, Haslam D. The obesity epidemic and its management. London: Matthew Wright: Pharmaceutical Press; 2010, pp. 264–7. doi: 10.1111/j.1365-2621.2010.02287.x
- Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford: Clarendon Press; 1989; pp. 20–30. doi: 10.1093/acprof:oso/9780198717478.001.0001
- Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OL. On the in vitro antioxidant properties of melatonin. J Pineal Res 2002; 3(3): 167–71. doi: 10.1034/j.1600-079X.2002.20920.x
- Han ZH, Ye JM, Wang GF. Evaluation of in vivo antioxidant activity of Hericium erinaceum polysaccharides. Int J Biol Macromol 2013; 2: 66–71. doi: 10.1016/j.ijbiomac.2012.09.009
- Carlsen MH, Halvorsen BL, Holte K, Bøhn SK, Dragland S, Sampson L, et al. The total antioxidant content of more than 3100 foods, beverage, spices, herbs and supplements used worldwide. Nutr J 2010; 9: 3. doi: 10.1186/1475-2891-9-3.
- Gentile D, Fornai M, Pellegrini C, Colucci R, Blandizzi C, Antonioli L. Dietary flavonoids as a potential intervention to improve redox balance in obesity and related comorbidities: a review. Nutr Res Rev 2018; 31(2): 239–247. doi: 10.1017/S0954422418000082
- Vogel O, Bart’s J, Qin M, Mitchell JD. Oriental lacquer. Progress in pacific polymer science. Vol. 3. Berlin: Springer; 1994, pp. 423–32. doi: 10.1007/978-3-642-78759-1_35
- Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY, et al. Rhus verniciflua stokes flavonoid extracts have anti-oxidant, anti-microbial and α-glucosidase inhibitory effect. Food Chem 2010; 120: 539–43. doi: 10.1016/j.foodchem.2009.10.051
- Song MY, Jeong GS, Kwon KB, Ka SO, Jang HY, Park JW, et al. Sulfuretin protects against cytokine-induced β-cell damage and prevents streptozotocin-induced diabetes. Exp Mol Med 2010; 42: 628–38. doi: 10.3858/emm.2010.42.9.062
- Hong DH, Han SB, Lee CW, Park SH, Jeon YJ, Kim MJ, et al. Cytotoxicity of urushiols isolated from sap of Korea lacquer tree (Rhus vernicifera Stokes). Arch Pharm Res 1999; 22: 638–41. doi: 10.1007/BF02975339
- Kim S, Park SE, Sapkota K, Kim MK, Kim SJ. Leaf extract of Rhus verniciflua stokes protects dopaminergic neuronal cells in a rotenone model of Parkinson’s disease. J Pharm Pharmacol 2011; 63: 1358–67. doi: 10.1111/j.2042-7158.2011.01342.x
- Hiruma W, Suruga K, Kadokura K, Tomita T, Miyata A, Sekino Y, et al. Antitumor effects and acute oral toxicity studies of a plant extract mixture containing Rhus verniciflua and some other herbs. Open J Immunol 2015; 5: 39–49. doi: 10.4236/oji.2015.51005
- Suruga K, Hiruma W, Kadokura K, Tomita T, Miyata A, Sekino Y, et al. Antitumour and apoptotic effects of a plant extract mixture containing Rhus verniciflua and other herbs in human leukaemia cells. Drug Res 2017; 65: 127–30. doi: 10.1055/s-0042-113458
- Clement Vicario F, Couto GC, Suruga K, Komatsu Y, Buffington CAT, Kisseberth WC. Clinical effects of a plant extract mixture containing Rhus verniciflua and other herbs in tumor bearing dogs. J Cancer Ther 2016; 7: 464–70. doi: 10.4236/jct.2016.77048
- Kadokura K, Suruga K, Tomita T, Hiruma W, Yamada M, Kobayashi A, et al. Novel urushiols with human immunodeficiency virus type 1 reverse transcriptase inhibitory activity from the leaves of Rhus verniciflua. J Natl Med 2015; 69: 148–53. doi: 10.1007/s11418-014-0871-7
- Kiyohara H, Matsumoto T, Yamada H. Combination effects of herbs in a multi-herbal formula: expression of Juzen-taiho-to’s immune-modulatory activity on the intestinal immune system. Evid Based Complement Alternat Med 2004; 1(1): 83–91. doi: 10.1093/ecam/neh004
- Du Y, Oshima R. Reverse-phase liquid chromatographic separation and identification of constituents of urushiol in the sap of the lac tree Rhus vernicifera. J Chromatogr 1984; 248: 463–73. doi: 10.1016/S0021-9673(01)87848-1
- Ballinger A, Peaking SR. Orlistat: its current status as an anti-obesity drug. Eur J Pharmacol 2002; 440: 109–17. doi: 10.1016/S0014-2999(02)01422-X
- Bohula EA, Wait SD, McGuire DK, Inzucchi SE, Kuder J, Im K, et al. Cardiovascular safety of lorcaserin in overweight and obese. N Engl J Med 2018; 379: 1107–17. doi: 10.1056/NEJMoa1808721
- Basemen DH, Van Gal LF. Progress and challenges in anti-obesity pharmacotherapy. Lancet Diabetes Endocrinol 2018; 6: 237–48. doi: 10.1016/S2213-8587(17)30236-X
- Morisset AS, Huot C, Legare D, Tchernof A. Circulating IL-6 concentrations and abdominal adipocyte isoproterenol-stimulated lipolysis in women. Obesity 2008; 16: 1487–92. doi: 10.1038/oby.2008.242
- Shirouchi B, Nagao K, Inoue N, Ohkubo T, Hibino H, Yanagita T. Effect of dietary omega 3 phosphatidylcholine on obesity-related disorders in obese Otsuka long-evans tokushima fatty rats. J Agric Food Chem 2008; 55: 7170–6. doi: 10.1021/jf071225x
- Bray GA. Drug treatment of obesity. Rev Endocr Metab Disord 2001; 2: 403–18. doi: 10.1023/A:1011808701117
- Mai TT, Thu NN, Tien PG, Chuyen NV. Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relalationships with polyphenol contents. J Nutr Sci Vitaminol 2007; 53: 267–76. doi: 10.3177/jnsv.53.267
- Rayne S, Mazza G. Biological activities of extracts from Sumac (Rhus spp): a review. Plant Foods Hum Nutr 2007; 62: 165–75. doi: 10.1007/s11130-007-0058-4
- Kim JS, Yang J, Kim MY. Alpha glucosidase inhibitory effect, anti-microbial activity and UPLC analysis of Rhus verniciflua under various extract conditions. J Med Plants Res 2011; 5(5): 778–83. doi: 10.5897/JMPR/E52792F18942
- Miyata M, Koyama T, Yazawa K. Water extract of Houttuynia cordata Thunb. Leaves exerts anti-obesity effects by inhibiting fatty acid and glycerol absorption. J Nutr Sci Vitaminol 2010; 56: 150–6. doi: 10.3177/jnsv.56.150
- Kawasumi K, Murai T, Mizorogi T, Okada Y, Yammoto I, Suruga K, et al. Changes in plasma metabolites concentrations in obese dogs supplemented with anti-oxidant compounds, Front Nutr 2018; 5: 74. doi: 10.3389/fnut.2018.00074
- Reaven GM. Role of insulin resistance in human disease. Diabetes 1988; 37: 1595–607. doi: 10.2337/diab.37.12.1595
- Farr OM, Gavrieli A, Mantzoros CS. Leptin applications in 2015: what have we learned about leptin and obesity? Curr Opin Endocrinol Diabetes Obes 2015; 22: 353–9. doi: 10.1097/MED.0000000000000184
- Heymsfield SB, Greenberg AS, Fujioka K, Dixon RM, Kushner R, Hunt T, et al. Recombinant leptin for weight loss in obese and lean adults: a randomized, controlled, dose-escalation trial. JAMA 1999; 282: 1568–76. doi: 10.1001/jama.282.16.1568
- Liu AG, Smith SR, Fujioka K, Greenway FL. The effect of leptin, caffeine/ephedrine, and their combination upon visceral fat mass and weight loss. Obesity 2013; 21: 1991–6. doi: 10.1002/oby.20416
- Pereira RP, Fachinetto R, Preste AS, Puntel RL, Silva GNS, Heinzmann BM, et al. Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem Res 2009; 34: 973–83. doi: 10.1007/sl1064-008-9861-z
- Bose S, Maji S, Chakraborty P. Quercitrin from Ixora coccinea leaves and its anti-oxidant activity. J Pharma Sci Tech 2013; 2: 72–4. Available from: https://www.researchgate.net/publication/281313923
- Panat NA, Amrute BK, Bhattu S, Haram SK, Shrma GK, Ghaskadbi SS. Antioxidant profiling of C3 quercetin glycosides: quercitrin, quercetin 3-β-D-glucoside and quercetin 3-O-(6’’-O-malonyl)-β-D-glucoside in cell free environment. Free Rad Antiox 2015; 5: 90–100. doi: 10.5530/fra.2015.2.7
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