Lycium barbarum polysaccharide attenuates Pseudomonas- aeruginosa pyocyanin-induced cellular injury in mice airway epithelial cells

  • Xue Lin Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Fuyang Song Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Yiming Wu Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Di Xue Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Yujiong Wang Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China
DOI: https://doi.org/10.29219/fnr.v66.4585
Keywords: Lycium barbarum polysaccharide (LBP), pyocyanin (PCN), air-liquid interface, ROS

Abstract

Background: Lycium barbarum berries have been utilized in Asia for many years. However, the mechanisms of its lung-defensive properties are indeterminate.

Objective: We investigate whether L. barbarum polysaccharide (LBP) could weaken Pseudomonas aeruginosa infection-induced lung injury.

Design: Mice primary air-liquid interface epithelial cultures were pretreated with LBP and subsequently treated with pyocyanin (PCN). Lung injury, including apoptosis, inflammation, and oxidative stress, was estimated by western blot, enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction, Real-time qPCR (Q-PCR). Flow cytometry was used to test cell apoptosis. Moreover, Balb/c mice were used to evaluate the tissue injury. We used hematoxylin-eosin staining and immunofluorescence to detect the expression of related proteins and tissue damage in mouse lungs and spleen.

Results: The flow cytometric analysis shows the potential of LBP to reduce time-dependent cell death by PCN. Mechanistically, LBP reduces PCN-induced expression of proapoptotic proteins and caspase3 and induces the activation of Bcl-2 in mice bronchial epithelial cells. Similarly, LBP reduces PCN-induced intracellular reactive oxygen species (ROS) production. Moreover, LBP inhibits the production of inflammatory cytokines, Interleukin (IL-1β), Tumor Necrosis Factor (TNF), IL-6, and IL-8. Our study confirms the ability of LBP to retard PCN-induced injury in mice lung and spleen.

Conclusions: The inhibition of PCN-induced lung injury by LBP is capable of protecting mice cells from injury.

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Published
2022-02-11
How to Cite
Lin X., Song F., Wu Y., Xue D., & Wang Y. (2022). <em>Lycium barbarum</em&gt; polysaccharide attenuates Pseudomonas- aeruginosa pyocyanin-induced cellular injury in mice airway epithelial cells. Food & Nutrition Research, 66. https://doi.org/10.29219/fnr.v66.4585
Issue
Vol 66 (2022)
Section
Original Articles
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