Lycium barbarum polysaccharide attenuates Pseudomonas- aeruginosa pyocyanin-induced cellular injury in mice airway epithelial cells

  • Xue Lin Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Fuyang Song Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Yiming Wu Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Di Xue Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, Ningxia, China
  • Yujiong Wang Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia, China
Keywords: Lycium barbarum polysaccharide (LBP), pyocyanin (PCN), air-liquid interface, ROS

Abstract

Background: Lycium barbarum berries have been utilized in Asia for many years. However, the mechanisms of its lung-defensive properties are indeterminate.

Objective: We investigate whether L. barbarum polysaccharide (LBP) could weaken Pseudomonas aeruginosa infection-induced lung injury.

Design: Mice primary air-liquid interface epithelial cultures were pretreated with LBP and subsequently treated with pyocyanin (PCN). Lung injury, including apoptosis, inflammation, and oxidative stress, was estimated by western blot, enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction, Real-time qPCR (Q-PCR). Flow cytometry was used to test cell apoptosis. Moreover, Balb/c mice were used to evaluate the tissue injury. We used hematoxylin-eosin staining and immunofluorescence to detect the expression of related proteins and tissue damage in mouse lungs and spleen.

Results: The flow cytometric analysis shows the potential of LBP to reduce time-dependent cell death by PCN. Mechanistically, LBP reduces PCN-induced expression of proapoptotic proteins and caspase3 and induces the activation of Bcl-2 in mice bronchial epithelial cells. Similarly, LBP reduces PCN-induced intracellular reactive oxygen species (ROS) production. Moreover, LBP inhibits the production of inflammatory cytokines, Interleukin (IL-1β), Tumor Necrosis Factor (TNF), IL-6, and IL-8. Our study confirms the ability of LBP to retard PCN-induced injury in mice lung and spleen.

Conclusions: The inhibition of PCN-induced lung injury by LBP is capable of protecting mice cells from injury.

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References


  1. Yan JG, Yi FW, Yu QW, Fang G, Zhi GC. Lycium Barbarum: a traditional chinese herb and a promising anti-aging agent. Aging Dis 2017; 8(6): 778. doi: 10.14336/AD.2017.0725

  2. Ming LJ, Qing SH, Ke Z, Peng S. Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L. Int J Biol Macromol 2013; 54: 16–23. doi: 10.1016/j.ijbiomac.2012.11.023

  3. Liu C, Liao J-Z, Li P-Y. Traditional Chinese herbal extracts inducing autophagy as a novel approach in therapy of nonalcoholic fatty liver disease. World J Gastroenterol 2017; 23(11): 1964. doi: 10.3748/wjg.v23.i11.1964

  4. Ma ZF, Zhang ZH, Teh SS, Wang CH, Zhan YT, Hayford F, et al. Goji Berries as a potential natural antioxidant medicine: an insight into their molecular mechanisms of action. Oxid Med Cell Longev 2019; 2019: 2437397.

  5. Masci A, Carradori S, Casadei MA, Paolicel P, Petralito S, Rangno R, et al. Lycium barbarum polysaccharides: extraction, purification, structural characterisation and evidence about hypoglycaemic and hypolipidaemic effects. A review. Food Chem 2018; 254: 377–89. doi: 10.1016/j.foodchem.2018.01.176

  6. Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol 2011; 19(8): 419–26. doi: 10.1016/j.tim.2011.04.005

  7. Chevalier S, Bouffaryigues E, Bodilis J, Maillot O, Lesouhaitie O, Feuilloley MJ, et al. Structure, function and regulation of Pseudomonas aeruginosa porins. FEMS Microbiol Rev 2017; 41(5): 698–722. doi: 10.1093/femsre/fux020

  8. Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 2009; 22(4): 582–610. doi: 10.1128/CMR.00040-09

  9. Rybtke M, Hultqvist LD, Givskov M, Nielsen TT. Pseudomonas aeruginosa biofilm infections: community structure, antimicrobial tolerance and immune response. J Mol Biol 2015; 427(23): 3628–45. doi: 10.1016/j.jmb.2015.08.016

  10. Schalk IJ, Cunrath O. An overview of the biological metal uptake pathways in P seudomonas aeruginosa. Environ Microbiol 2016; 18(10): 3227–46. doi: 10.1111/1462-2920.13525

  11. Valentini M, Gonzalez D, Mavridou DA. Lifestyle transitions and adaptive pathogenesis of Pseudomonas aeruginosa. Curr Opin Microbiol 2018; 41: 15–20. doi: 10.1016/j.mib.2017.11.006

  12. Lin H, Li H, Cho HJ, Bian S, Roh HJ. Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci 2007; 96(2): 341–50. doi: 10.1002/jps.20803

  13. Xue D, Li Y, Jiang Z, Deng G, Li M, Liu X, Wang YJ. A ROS-dependent and Caspase-3-mediated apoptosis in sheep bronchial epithelial cells in response to Mycoplasma ovipneumoniae infections. Vet Immunol Immunopathol 2017; 187: 55–63. doi: 10.1016/j.vetimm.2017.04.004

  14. Curran CS, Bolig T, Torabi-Parizi P. Mechanisms and targeted therapies for Pseudomonas aeruginosa lung infection. Am J Respir Crit Care Med 2018; 197(6): 708–27. doi: 10.1164/rccm.201705-1043SO

Published
2022-02-11
How to Cite
LinX., SongF., WuY., XueD., & WangY. (2022). <em>Lycium barbarum</em&gt; polysaccharide attenuates Pseudomonas- aeruginosa pyocyanin-induced cellular injury in mice airway epithelial cells. Food & Nutrition Research, 66. https://doi.org/10.29219/fnr.v66.4585
Section
Original Articles