Dietary lactoferrin has differential effects on gut microbiota in young versus middle-aged APPswe/PS1dE9 transgenic mice but no effects on cognitive function

  • Huan-Huan Zhou Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China
  • Guiping Wang School of Physical Education, Soochow University, Suzhou, China; and Laboratory Animal Center, Medical College of Soochow University, Suzhou, China
  • Lan Luo Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China
  • Wei Ding Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China
  • Jia-Ying Xu School of Radiation Medicine and Protection, Soochow University, Suzhou, China
  • Zengli Yu School of Public Health, Zhengzhou University, Zhengzhou, China
  • Li-Qiang Qin Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China
  • Zhongxiao Wan Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China; and School of Public Health, Zhengzhou University, Zhengzhou, China
Keywords: Alzheimer’s disease; lactoferrin; cognitive function; gut microbiota; amyloid β


Background: Existing evidence suggest that lactoferrin might be beneficial for Alzheimer’s disease, while precise mechanisms are not fully elucidated.

Objective: To determine the effects of lactoferrin intervention on cognitive function from APPswe/PS1dE9 (APP/PS1) mice, and potential mechanisms involved.

Design: Both the young and middle-aged male APP/PS1 mice were divided into the control and lactoferrin intervention groups with 16 weeks’ intervention.

Results: Lactoferrin had no effects on cognitive function for both the young and middle-aged mice, and no key markers involved in Aβ, tau pathology, neuro-inflammation and synaptic plasticity were altered after lactoferrin intervention. With regards to gut microbiota profiles, in the young APP/PS1 mice, lactoferrin elevated the α diversity index including ACE and Chao 1, and reduced the relative abundance of the genera Bacteroides and Alistipes and elevated Oscillibacter; in addition, Oscillibacter, Anaerotruncus, EF096579_g, EU454405_g, Mollicutes_RF39, EU474361_g, EU774448_g, and EF096976_g were specifically abundant via linear discriminant analysis with effect size (LEfSe) analysis. In the middle-aged APP/PS1 mice, the relative abundance of the phylum Proteobacteria, as well as the genera Oscillospira, Coprococcus, and Ruminococcus was significantly reduced post lactoferrin; additionally, S24_7BacteroidiaBacteroidetes, and Methylobacterium were specific via LEfSe analysis in the lactoferrin group.

Conclusions: Dietary lactoferrin might be beneficial for gut microbiota homeostasis although it might have no effects on cognition.


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How to Cite
Zhou, H.-H., Wang, G., Luo, L., Ding, W., Xu, J.-Y., Yu, Z., Qin, L.-Q., & Wan, Z. (2021). Dietary lactoferrin has differential effects on gut microbiota in young versus middle-aged APPswe/PS1dE9 transgenic mice but no effects on cognitive function. Food & Nutrition Research, 65.
Original Articles