Clusterin levels in undernourished SH-SY5Y cells

  • Carmen Rodríguez-Rivera Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid, Spain
  • María Dolores Pérez-Carrión Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid; and Facultad de Medicina, Universidad de Castilla-la Mancha, Albacete, Spain
  • Lucía Casariego Olavarría Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid, Spain
  • Luis F. Alguacil Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid; and Facultad de Farmacia, Instituto de Estudio de las Adicciones, Universidad CEU San Pablo, Alcorcón, Madrid, Spain
  • María José Polanco Mora Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid; and Facultad de Farmacia, Instituto de Estudio de las Adicciones, Universidad CEU San Pablo, Alcorcón, Madrid, Spain
  • Carmen González-Martín Facultad de Farmacia, Universidad CEU San Pablo, Alcorcón, Madrid; and Facultad de Farmacia, Instituto de Estudio de las Adicciones, Universidad CEU San Pablo, Alcorcón, Madrid, Spain
Keywords: clusterin, food addiction, undernutrition, mitochondria, cell survival


Food-related disorders are increasingly common in developed societies, and the psychological component of these disorders has been gaining increasing attention. Both overnourishment with high-fat diets and perinatal undernourishment in mice have been linked to a higher motivation toward food, resulting in an alteration in food intake. Clusterin (CLU), a multifaced protein, is overexpressed in the nucleus accumbens (NAc) of overfed rats, as well as in those that suffered chronic undernutrition. Moreover, an increase of this protein was observed in the plasma of obese patients with food addiction, suggesting the implication of CLU in this eating disorder. To characterize CLU’s cellular mechanisms, in vitro experiments of undernutrition were performed using dopaminergic SH-SY5Y cells. To mimic in vivo dietary conditions, cells were treated with different fetal bovine serum (FBS) concentrations, resulting in control (C group) diet (10% FBS), undernourishment (U group) diet (0.5% FBS), and undernourishment diet followed by restoration of control diet (UC group) (0.5 + 10% FBS). Undernourishment compromised cell viability and proliferation, and concomitantly increased CLU secretion as well as the cytosolic pool of the protein, while decreasing the mitochondrial level. The restoration of normal conditions tended to recover cell physiology, and the normal levels and distribution of CLU. This research study is a step forward toward the characterization of clusterin as a potential marker for food addiction and nutritional status.


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How to Cite
Rodríguez-Rivera, C., Pérez-Carrión, M. D., Casariego Olavarría, L., Alguacil, L. F., Polanco Mora, M. J., & González-Martín, C. (2021). Clusterin levels in undernourished SH-SY5Y cells. Food & Nutrition Research, 65.
Original Articles