The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells

  • Min Sun Tianjin University of Science & Technology
  • Xiaoling Lu Tianjin University of Science & Technology
  • Lei Hao Tianjin University of Science & Technology
  • Tao Wu Tianjin University of Science & Technology
  • Huanjiao Zhao Tianjin University of Science & Technology
  • Chao Wang Tianjin University of Science & Technology
Keywords: Food composition

Abstract

Background: Anthocyanins have been proven to be beneficial to the eyes. However, information is scarce about the effects of purple sweet potato (Ipomoea batatas, L.) anthocyanin (PSPA), a class of anthocyanins derived from purple sweet potato roots, on visual health.

Objective: The aim of this study was to investigate whether PSPA could have influences on the growth characteristics (cellular morphology, survival, and proliferation) of human retinal pigment epithelial (RPE) cells, which perform essential functions for the visual process.

Methods: The RPE cell line D407 was used in the present study. The cytotoxicity of PSPA was assessed by MTT assay. Then, cellular morphology, viability, cell cycle, Ki67expression, and PI3K/MAPK activation of RPE cells treated with PSPA were determined.

Results: PSPA exhibited dose-dependent promotion of RPE cell proliferation at concentrations ranging from 10 to 1,000 µg/ml. RPE cells treated with PSPA demonstrated a predominantly polygonal morphology in a mosaic arrangement, and colony-like cells displayed numerous short apical microvilli and typical ultrastructure. PSPA treatment also resulted in a better platform growing status, statistically higher viability, an increase in the S-phase, and more Ki67+ cells. However, neither pAkt nor pERK were detected in either group.

Conclusions: We found that PSPA maintained high cell viability, boosted DNA synthesis, and preserved a high percentage of continuously cycling cells to promote cell survival and division without changing cell morphology. This paper lays the foundation for further research about the damage-protective activities of PSPA on RPE cells or human vision.

Keywords: PSPA; RPE cells; morphology; survival; proliferation

(Published: 11 June 2015)

Citation: Food & Nutrition Research 2015, 59: 27830 - http://dx.doi.org/10.3402/fnr.v59.27830

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Author Biographies

Min Sun, Tianjin University of Science & Technology
Department of  Food Sciences & Bioengineering?Doctor
Xiaoling Lu, Tianjin University of Science & Technology
Department of  Food Sciences & Bioengineering?Professor
Lei Hao, Tianjin University of Science & Technology

Department of  Food Sciences & Bioengineering?Doctor

Tao Wu, Tianjin University of Science & Technology

Department of  Food Sciences & Bioengineering?Doctor

Huanjiao Zhao, Tianjin University of Science & Technology
Department of  Food Sciences & Bioengineering
Chao Wang, Tianjin University of Science & Technology
Department of  Food Sciences & Bioengineering
Published
2015-06-11
How to Cite
Sun, M., Lu, X., Hao, L., Wu, T., Zhao, H., & Wang, C. (2015). The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells. Food & Nutrition Research, 59. https://doi.org/10.3402/fnr.v59.27830
Section
Original Articles

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