The effect of extra virgin olive oil on HSP27 expression in the cerebral cortex of male Rattus norvegicus middle cerebral artery occlusion model

Abdulloh Machin; Viskasari Pintoko Kalanjati; Anisa Nur’aini Abidah; paulus sugianto; Joni Susanto; Azizah Amimathul Firdha

doi:10.29219/fnr.v68.9500

Abdulloh Machin Departement of Neurology, Faculty of Medicine, Universitas Airlangga
Viskasari Pintoko Kalanjati Departement of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Anisa Nur’aini Abidah Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
paulus sugianto Departement of Neurology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Joni Susanto Departement of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Azizah Amimathul Firdha Indonesian Medical Association (IDI) Surabaya chapter, Surabaya, INDONESIA

DOI: https://doi.org/10.29219/fnr.v68.9500

Keywords: evoo, olive oil, HSP27, MCAO, stroke

Abstract

Background: Ischemic stroke produces oxidative stress in neurons and neuroglial, triggering a cellular response with HSP27 as one of the markers. Extra virgin olive oil (EVOO) is reported to act as a neuroprotection by inhibiting reactive oxygen species, which can modulate HSP27.

Objective: To analyze the potential neuroprotection of EVOO containing 7-g MUFA on the expression of HSP27 in the cerebral cortex of Rattus norvegicus Wistar strain middle cerebral artery occlusion (MCAO) model.

Design: MCAO-Rattus norvegicus Wistar male rodents (n = 32), randomly divided into negative control (sham), positive control (P0), MCAO added EVOO 0.5 mL/kg body weight (P1), and MCAO added EVOO 2 mL/kg body weight (P2); the experiment was conducted 14 days. The nuclei of neurons, glial, and expression of HSP27 on the cerebral cortex were analyzed using Image J software, data were compared between groups with a significance level of P < 0.05.

Results: There were significant differences in the neuron and glia nuclei numbers between groups (P = 0.01). There was also a significant difference in cerebral cortex HSP27 expression between groups (P = 0.006).

Conclusion: There was a significant down-regulated of HSP27 in the cerebral cortex of the male Rattus norvegicus Wistar strain MCAO model given EVOO with 7 g of MUFA content at a dose of 0.5–2 mL/kgBW.

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