Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells

  • Gi-Chang Kim
  • Jin-Sook Kim
  • Gyoung-Mi Kim
  • Song-Yi Choi
Keywords: Edible flower, obesity, PPARG, SREBF1, CEBPA


Background : Edible flowers, Tropaeolum majus has been used as a disinfectant and an antibiotic, and for wound healing, but the anti-obesity effects of this plant have not been reported previously

Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells.

Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid accumulation levels were determined using Oil-Red O staining and a triglyceride content assay. Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting.

Results : The highest inhibition of lipid accumulation was observed at a TME concentration of 300 µg/mL. Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. This decrease was shown to be concentration-dependent.

Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells.

Conclusions : TME may be a potential novel therapeutic agent for the prevention and treatment of obesity.


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How to Cite
Kim G.-C., Kim J.-S., Kim G.-M., & Choi S.-Y. (2017). Anti-adipogenic effects of <em>Tropaeolum majus</em&gt; (nasturtium) ethanol extract on 3T3-L1 cells. Food & Nutrition Research, 61. Retrieved from
Original Articles