Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats

  • Mao-Cheng Sun
  • Ping-Ping Hou
  • Xin-Yu Wang
  • Chang-Hui Zhao
  • Bi-Jun Cheng
  • Yan-Ling Wang
  • Hong-Wei Hao
  • Tie-Hua Zhang
  • Hai-Qing Ye
Keywords: Lactobacillus reuteri; ethanol; gastric injury; inflammatory; antioxidant


Background: Previous studies suggested that probiotics intervention may be one of the methods for preventing and/or treating gastric ulcer.

Objective: The aim of the study was to compare the preventive effects of a spaceflight mutant Lactobacillus reuteri F-9-35 and its wild type on ethanol-induced gastric injury in rats.

Design: Forty rats were randomly allocated into five groups: a normal group (NOR), ethanol group (EtOH), skim milk group (MILK), L. reuteri F-9-35 group (F935) and wild-type group (WT). The NOR and EtOH groups received 1 ml of distilled water by daily gavage for 14 days. The MILK group received 1 ml of skim milk alone, while the F935 and WT groups were administered 1 ml of skim milk containing the mutant and wild type (1 × 1010 colony-forming unit/ml) by daily gavage for 14 days, respectively. Acute gastric injury was induced by absolute alcohol 1 h after the final administration of different treatments, except for the NOR group.

Results: Pretreatment with L. reuteri F-9-35, but not milk alone or milk with the L. reuteri wild type, showed significant reduction of ethanol-induced gastric injury, as evidenced by lowering of ulcer index, ulcer area (%), and histological lesion. F-9-35 decreased the levels of lipid peroxidation and myeloperoxidase and increased mucus, glutathione, and nitric oxide levels in gastric tissue. Moreover, F-9-35 inhibited the expression of proinflammatory genes including gastric tumor necrosis factor-α, interleukin-1β, and cyclooxygenase-2 and decreased the activity of nuclear factor kappa B (NF-κB).

Conclusion: These findings indicated that L. reuteri F-9-35 pretreatment can attenuate ethanol-induced gastric injury in rats by inhibiting oxidative stress and inflammatory response. Together, L. reuteri F-9-35 has potential preventive efficacy on gastric ulcer.


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How to Cite
Sun M-C, Hou P-P, Wang X-Y, Zhao C-H, Cheng B-J, Wang Y-L, Hao H-W, Zhang T-H, Ye H-Q. Pretreatment with <em>Lactobacillus reuteri</em&gt; F-9-35 attenuates ethanol-induced gastric injury in rats. fnr [Internet]. 2018Oct.29 [cited 2018Nov.19];620. Available from:
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