Inclusion of microbe-derived antioxidant during pregnancy and lactation attenuates high-fat diet-induced hepatic oxidative stress, lipid disorders, and NLRP3 inflammasome in mother rats and offspring

  • Zhen Luo
  • Xue Xu
  • Sen Zhao
  • Takami Sho
  • Wenli Luo
  • Jing Zhang
  • Weina Xu
  • Kong Hon
  • Jianxiong Xu
Keywords: high fat diet; Liver; microbiota-fermented antioxidants; NLRP3 inflammasome; rats


Objective: This study aimed to evaluate the effects of microbe-derived antioxidant (MA) on high-fat diet (HFD)-induced hepatic lipid disorders in mother rats and offspring.

Methods: A total of 36 female rats were randomly divided into three groups at the beginning of pregnancy: the control group (CG), HFD, and HFD with 2% MA. Mother rats were slaughtered at the first and 10th day of lactation (L1 and L10) and offspring were slaughtered at L10. The plasma and liver of mother rats, and liver of offspring were collected.

Results: The results showed that MA reversed HFD-induced activities of inducible nitric oxide synthase (iNOS) and antioxidative enzymes in liver of mother rats and offspring. In addition, MA reduced HFD-induced lipid accumulation through decreasing the low-density lipoprotein cholesterol (LDLC) content in plasma of mother rats and improving hepatic fatty acid synthase (FAS) in mother rats and offspring. MA decreased HFD-induced hepatic alkaline phosphatase (AKP) activity in liver of mother rats and offspring. Furthermore, MA reduced HFD-activated nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome in liver of mother rats and offspring.

Conclusions: MA supplementation reversed HFD-induced hepatic oxidative stress, lipid accumulation, NLRP3 inflammasome, and function in mother rats and offspring, suggesting MA can be functional ingredients to improve maternal-fetal health.


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Author Biographies

Zhen Luo

school of agriculture and biology

Xue Xu

school of agriculture and biology

Sen Zhao

school of agriculture and biology

Takami Sho

school of agriculture and biology

Wenli Luo

school of agriculture and biology

Jing Zhang

school of agriculture and biology

Weina Xu

school of agriculture and biology


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How to Cite
Luo Z, Xu X, Zhao S, Sho T, Luo W, Zhang J, Xu W, Hon K, Xu J. Inclusion of microbe-derived antioxidant during pregnancy and lactation attenuates high-fat diet-induced hepatic oxidative stress, lipid disorders, and NLRP3 inflammasome in mother rats and offspring. fnr [Internet]. 2019Aug.23 [cited 2019Sep.16];630. Available from:
Original Articles