Anti-diabetic effects of the soluble dietary fiber from tartary buckwheat bran in diabetic mice and their potential mechanisms

  • Weijing Wu Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen, Fujian, and Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian, China
  • Zaigui Li College of Food Science and Nutritional Engineering, China Agricultural University, Haidian, Beijing, China
  • Fei Qin Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen, Fujian, China
  • Ju Qiu Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Haidian, Beijing, China
Keywords: Soluble dietary fiber, tartary buckwheat, glucose metabolism, lipid metabolism, short-chain fatty acids, AMP-activated protein kinase

Abstract

Background: Tartary buckwheat has beneficial effects on glucose and lipid metabolism of patients with type 2 diabetes mellitus. However, the physiological effects of a soluble dietary fiber (SDF) from tartary buckwheat have rarely been studied, especially in vivo.

Objective: This study aimed to examine the hypoglycemic and hypolipidemic effects of SDF from tartary buckwheat bran on high-fat diet/streptozotocin-induced diabetic mice.

Design: The SDF of tartary buckwheat bran was collected according to the Association of Official Analytical Chemists method 991.43. Diabetic mice were treated with high-fat diets supplemented with 0.5, 1, and 2% SDF for 8 weeks. Parameters related to glucose and lipid metabolism and relevant mechanisms, including the excretion of short-chain fatty acids and the glycemic signaling pathway in the liver, were investigated. In addition, the structural characterization of a purified polysaccharide from SDF of tartary buckwheat bran was illustrated.

Result: Supplementation with SDF in the diet resulted in reduced levels of fasting blood glucose, improved oral glucose tolerance, increased levels of liver glycogen and insulin, as well as improved lipid profiles in both the serum and liver, in diabetic mice. The amelioration of glucose and lipid metabolism by SDF was accompanied by an increase in the short-chain fatty acid levels in the cecum and co-regulated by hepatic adenosine- 5′-monophosphate-activated protein kinase (AMPK) phosphorylation. A neutral tartary buckwheat polysaccharide with an average molecular weight of 19.6 kDa was purified from the SDF, which consisted mainly of glucose with α-glycosidic bonds.

Conclusions: The SDF of tartary buckwheat bran exhibits hypoglycemic and hypolipidemic effects in diabetic mice, contributing to the anti-diabetic mechanisms of tartary buckwheat.

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Published
2021-01-08
How to Cite
Wu, W., Li, Z., Qin, F., & Qiu, J. (2021). Anti-diabetic effects of the soluble dietary fiber from tartary buckwheat bran in diabetic mice and their potential mechanisms. Food & Nutrition Research, 65. https://doi.org/10.29219/fnr.v65.4998
Section
Original Articles