D-allulose ameliorates adiposity through the AMPK-SIRT1-PGC-1α pathway in HFD-induced SD rats

  • Geum Hwa Lee Non-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju
  • Cheng Peng Non-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju
  • Hwa-Young Lee Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju
  • Seon-Ah Park Non-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju
  • The-Hiep Hoang Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju
  • Jung Hyun Kim Department of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju
  • Soonok Sa Samyang Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do
  • Go-Eun Kim Samyang Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do
  • Jung-Sook Han Samyang Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do
  • Han Jung Chae Non-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital
DOI: https://doi.org/10.29219/fnr.v65.7803
Keywords: D-allulose; obesity; adipose tissue; AMPK; SIRT1.

Abstract

Background: Adiposity is a major health-risk factor, and D-allulose has beneficial effects on adiposity-related metabolic disturbances. However, the modes of action underlying anti-hyperglycemic and hypolipidemic activity are partly understood.

Objective: This study investigated the in vivo and in vitro effects of D-allulose involved in adipogenesis and activation of the AMPK/SIRT1/PGC-1α pathway in high-fat diet (HFD)-fed rats.

Design: In this study, 8-week-old male SD (Sprague Dawley) rats were divided into five groups (n = 8/group), (1) Control (chow diet, 3.5%); (2) 60% HFD; (3) 60% HFD supplemented with allulose powder (AP) at 0.4 g/kg; (4) 60% HFD supplemented with allulose liquid (AL) at 0.4 g/kg; (5) 60% HFD supplemented with glucose (AL) at 0.4 g/kg. All the group received the product through oral gavage for 6 weeks. Control and HFD groups were gavaged with double-distilled water.

Results: Rats receiving AP and AL showed reduced body weight gain and fat accumulation in HFD-fed rats. Also, supplementation of AL/AP regulated the cytokine secretion and recovered biochemical parameters to alleviate metabolic dysfunction and hepatic injury. Additionally, AL/AP administration improved adipocyte differentiation via regulation of the PPARγ and C/EBPα signaling pathway and adipogenesis-related genes owing to the combined effect of the AMPK/SIRT1 pathway. Furthermore, AL/AP treatment mediated PGC-1α expression triggering mitochondrial genesis via activating the AMPK phosphorylation and SIRT1 deacetylation activity in adipose tissue.

Conclusion: The anti-adiposity activity of D-allulose is observed on a marked alleviation in adipogenesis and AMPK/SIRT1/PGC-1α deacetylation in the adipose tissue of HFD-fed rat.

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Published
2021-12-21
How to Cite
Lee G. H., Cheng Peng, Lee H.-Y., Park S.-A., Hoang T.-H., Kim J. H., Sa S., Kim G.-E., Han J.-S., & Chae H. J. (2021). D-allulose ameliorates adiposity through the AMPK-SIRT1-PGC-1α pathway in HFD-induced SD rats. Food & Nutrition Research, 65. https://doi.org/10.29219/fnr.v65.7803
Issue
Vol 65 (2021)
Section
Original Articles
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