Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux
Abstract
Background: Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease (CVD).
Objective: This study investigated the protective effects of resveratrol against hydrogen peroxide-induced damage in cardiomyocytes.
Design: In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts were studied at an increasing concentration from 0 to 100 µM.
Results: Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h of administration.
Conclusions: These results suggest that resveratrol-regulated autophagy may play a role in degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a possible mechanism by which resveratrol protects the heart during CVD.
Keywords: resveratrol; oxidative stress; apoptosis; autophagy
(Published: 20 May 2016)
Citation: Food & Nutrition Research 2016, 60: 30511 - http://dx.doi.org/10.3402/fnr.v60.30511
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