Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux

  • Chih-Yang Huang
  • Wei-Jen Ting
  • Chih-Yang Huang
  • Jing-Yi Yang
  • Wan-Teng Lin


Background: Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease (CVD).

Objective: This study investigated the protective effects of resveratrol against hydrogen peroxide-induced damage in cardiomyocytes.

Design: In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts were studied at an increasing concentration from 0 to 100 µM.

Results: Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h of administration.

Conclusions: These results suggest that resveratrol-regulated autophagy may play a role in degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a possible mechanism by which resveratrol protects the heart during CVD.

Keywords: resveratrol; oxidative stress; apoptosis; autophagy

(Published: 20 May 2016)

Citation: Food & Nutrition Research 2016, 60: 30511 -


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How to Cite
Huang C.-Y., Ting W.-J., Huang C.-Y., Yang J.-Y., & Lin W.-T. (2016). Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux. Food & Nutrition Research, 60.
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