Effect of the extraction solvent and method on the determination of the total polyphenol content in different common buckwheat (Fagopyrum esculentum Moench) varieties

Pavlína Podloucká; Ivana Polišenská; Ondřej Jirsa

doi:10.29219/fnr.v69.9834

Pavlína Podloucká Agrotest Fyto, Ltd., Kroměříž, Czech Republic
Ivana Polišenská Agrotest Fyto, Ltd., Kroměříž, Czech Republic
Ondřej Jirsa Agrotest Fyto, Ltd., Kroměříž, Czech Republic

DOI: https://doi.org/10.29219/fnr.v69.9834

Keywords: total polyphenol content, buckwheat, solvent extraction, acid hydrolysis, alkaline hydrolysis

Abstract

Buckwheat is a pseudocereal whose seeds are rich in numerous health-positive phytochemicals including polyphenols. Several methods for extracting these compounds can be found in the literature. The objective of the study was to compare the total polyphenol content (TPC) of seven common buckwheat (Fagopyrum esculentum Moench) varieties obtained using different extraction methods/procedures and to assess the impact of the extraction methods on various varieties. The Folin–Ciocalteu spectrophotometric assay was used to measure the TPC. The results showed that TPC was significantly dependent on the extraction solvent and the efficiency of the solvents may be ordered from high to low efficacy as follows: 80% acetone > 0.1% HCl in methanol > 80% methanol = 80% ethanol > 100% methanol > water = 100% ethanol. TPC increased with increasing temperature during extraction and procedures based on alkaline hydrolysis proved to be more effective than those based on the acidic one. The responses of different buckwheat varieties to various extract preparations slightly differed, which could be attributed to each variety’s specific composition of extractable and bound polyphenols. It can be suggested that using more than one extraction method gives more robust information for good characterizing of buckwheat varieties according to their TPC for breeding and food use purposes.

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References

1.
Trouillas P, Marsal P, Siri D, Lazzaroni R, Duroux JL. A DFT study of the reactivity of OH groups in quercetin and taxifolin antioxidants: the specificity of the 3-OH site. Food Chem 2006; 97(4): 679–88. doi: 10.1016/j.foodchem.2005.05.042

2.
Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20(7): 933–56. doi: 10.1016/0891-5849(95)02227-9

3.
Scalbert A, Manach C, Morand C, Rémésy C, Jiménez L. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr 2005; 45(4): 287–306. doi: 10.1080/1040869059096

4.
Arts ICW, Hollman PC. Polyphenols and disease risk in epidemiologic studies. AJCN 2005; 81(1): 317S–25S. doi: 10.1093/ajcn/81.1.317S

5.
Shahidi F, Naczk M. Phenolics in food and nutraceuticals. Boca Raton, FL: CRC Press; 2003.

6.
Zhou K, Yu L. Effect of Extraction Solvent On Wheat Bran Antioxidant Activity Estimation. LWT – Food Sci Technol 2004; 37(7): 717–21. doi: 10.1016/j.lwt.2004.02.008

7.
Przybylski R, Lee YC, Eskin NAM. Antioxidant and radical-scavenging activities of buckwheat seed components J Am Oil Chem Soc 1998; 75(11): 1595–601. doi: 10.1007/s11746-998-0099-3

8.
Naczk M, Shahidi F. Extraction and analysis of phenolics in food. J Chromatogr A 2004; 1054(1–2): 95–111. doi: 10.1016/j.chroma.2004.08.059

9.
Inglett GE, Rose DJ, Chen D, Stevenson DG, Biswas A. Phenolic content and antioxidant activity of extracts from whole buckwheat (Fagopyrum esculentum Moench) with or without microwave irradiation. Food Chem 2010; 119(3): 1216–9. doi: 10.1016/j.foodchem.2009.07.041

10.
Holasova M, Fiedlerova V, Smrcinova H, Orsak M, Lachman J, Vavreinova S. Buckwheat – the source of antioxidant activity in functional foods. Food Res Int 2002; 35(2–3): 207–11. doi: 10.1016/S0963-9969(01)00185-5

11.
Rothwell JA, Pérez-Jiménez J, Neveu V, Medina-Ramon A, M’Hiri N, Garcia Lobato P, et al. Phenol-Explorer 3.0: a major update of the Phenol-Explorer database to incorporate data on the effects of food processing on polyphenol content. Database 2013; bat070. doi: 10.1093/database/bat070

12.
Jing R, Li HQ, Hu CL, Jiang YP, Qin LP, Zheng CJ. Phytochemical and pharmacological profiles of three Fagopyrum buckwheat. Int J Mol Sci 2016; 17(4): 589. doi: 10.3390/ijms17040589

13.
Kozlowska A, Szostak-Wegierek D. Flavonoids-food sources and health benefits. Rocz Panstw Zakl Hig 2014; 65(2): 79–85.

14.
Eliášová M, Paznocht L. Total phenolic content and antioxidant activity of tritordeum wheat and barley. Agron Res 2017; 15(S2): 1287–94.

15.
Stratil P, Klejdus B, Kubáň V. Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. J Agric Food Chem 2006; 54(3): 607–16. doi: 10.1021/jf052334j

16.
Singleton VL, Vernon L, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16(3): 144–58. doi: 10.5344/ajev.1965.16.3.144

17.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 1999; 299: 152–78. doi: 10.1016/S0076-6879(99)99017-1

18.
Sun T, Ho CT. Antioxidant activities of buckwheat extracts. Food Chem 2005; 90(4): 743–9. doi: 10.1016/j.foodchem.2004.04.035

19.
Inglett GE, Chen D, Berhow M, Lee S. Antioxidant activity of commercial buckwheat flours and their free and bound phenolic compositions. Food Chem 2011; 125(3): 923–9. doi: 10.1016/j.foodchem.2010.09.076

20.
Zieliński H, Kozłowska H. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J Agric Food Chem 2000; 48(6): 2008–16. doi: 10.1021/jf990619o

21.
Xu Y, Zhang R, Fu H. Studies on the optimal process to extract flavonoids from red-raspberry fruits. Nat Sci 2005; 3(2): 43–6.

22.
Hodzic Z, Pasalic H, Memisevic A, Srabovic M, Saletovic M, Poljakovic M. The influence of total phenols content on antioxidant capacity in the whole grain extracts. Eur J Sci Res 2009; 28(3): 471–7.

23.
Pérez-Jiménez J, Torres JL. Analysis of nonextractable phenolic compounds in foods: the current state of the art. J Agric Food Chem 2011; 59(24): 12713–24. doi: 10.1021/jf203372w

24.
Martín-García B, Pasini F, Verardo V, Gómez-Caravaca AM, Marconi E, Caboni MF. Distribution of free and bound phenolic compounds in buckwheat milling fractions. Foods 2019; 8(12): 670. doi: 10.3390/foods8120670

25.
Adom KK, Liu RH. Antioxidant activity of grains. J Agric Food Chem 2002; 50(21): 6182–7. doi: 10.1021/jf0205099

26.
Arranz S, Saura-Calixto F, Shaha S, Kroon PA. High contents of nonextractable polyphenols in fruits suggest that polyphenol contents of plant foods have been underestimated. J Agric Food Chem 2009; 57(16): 7298–303. doi: 10.1021/jf9016652

27.
Pérez-Jiménez J, Saura-Calixto F. Literature data may underestimate the actual antioxidant capacity of cereals. J Agric Food Chem 2005; 53(12): 5036–40. doi: 10.1021/jf050049u

28.
Lu L, Murphy K, Baik BK. Genotypic variation in nutritional composition of buckwheat groats and husks. Cereal Chem 2013; 90(2): 132–7. doi: 10.1094/CCHEM-07-12-0090-R

29.
Vollmannova A, Margitanova E, Tóth T, Timoracka M, Urminska D, Bojňanská T, et al. Cultivar influence on total polyphenol and rutin contents and total antioxidant capacity in buckwheat, amaranth, and quinoa seeds. Czech J Food Sci 2013; 31(6): 589–95. doi: 10.17221/452/2012-CJFS

30.
Domingos IF, Bilsborrow PE. The effect of variety and sowing date on the growth, development, yield and quality of common buckwheat (Fagopyrum esculentum Moench). Eur J Agron 2021; 126: 126264. doi: 10.1016/j.eja.2021.126264

31.
Oomah BD, Mazza G. Flavonoids and antioxidative activities in buckwheat. J Agric Food Chem 1996; 44(7): 1746–50. doi: 10.1021/jf9508357

32.
Guo XD, Ma YJ, Parry J, Gao JM, Yu LL, Wang M. Phenolics content and antioxidant activity of Tartary buckwheat from different locations. Molecules 2011; 16(12): 9850–67. doi: 10.3390/molecules16129850

33.
Ghiselli L, Tallarico R, Mariotti M, Romagnoli S, Baglio AP, Donnarumma P, et al. Agronomic and nutritional characteristics of three buckwheat cultivars under organic farming in three environments of the Garfagnana mountain district. Ital J Agron 2016; 11(3): 188–94. doi: 10.4081/ija.2016.729