Cytoprotective mechanism of ferulic acid against high glucose-induced oxidative stress in cardiomyocytes and hepatocytes

  • Yuan Song Jinan Univetsity
  • Luona Wen
  • Jianxia Sun
  • Weibin Bai Jinan University
  • Rui Jiao
  • Yunfeng Hu
  • Xichun Peng
  • Yong He
  • Shiyi Ou
Keywords: Molecular nutrition, ferulic acid, oxidative stress, cardiomyocytes, hepatocytes

Abstract

Background: Ferulic acid (FA), a phenolic acid, is a potential therapy for diabetes mellitus. FA has been shown to protect against hepatic and myocardial injury and oxidative stress in obese rats with late-stage diabetes, but the mechanism of the antioxidative activity of FA is still unclear.

Objective: The aim of this study was to elucidate whether FA can prevent damage to cardiomyocytes and hepatocytes caused by high glucose (HG)-induced oxidative stress and whether the protection effects of FA on these cells are related to the Keap1-Nrf2-ARE signaling pathways.

Design: Cells were divided into four groups: a control group (cultured with normal medium), an HG group (medium containing 80 mmol/L glucose), an FA+HG group (medium containing 80 mmol/L glucose and 1, 5, or 10 µg/mL FA), and a dimethylbiguanide (DMBG)+HG group (medium containing 80 mmol/L glucose and 50 µg/mL DMBG).

Results: FA treatment significantly increased cell viability and significantly decreased cell apoptosis compared with the HG-treated group. Moreover, FA down-regulated the expression of Keap1 protein and up-regulated the expression of Nrf2 protein and gene transcription of HO-1 and glutathione S-transferase (GST) in a dose-dependent manner.

Conclusion: FA alleviated the HG-induced oxidative stress and decreased cell apoptosis in hepatocytes and cardiomyocytes. These effects were associated with the Keap1-Nrf2-ARE signaling pathway.

Keywords: ferulic acid; oxidative stress; cardiomyocytes; hepatocytes

(Published: 10 February 2016)

Citation: Food & Nutrition Research 2016, 60: 30323 - http://dx.doi.org/10.3402/fnr.v60.30323

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Published
2016-02-10
How to Cite
1.
Song Y, Wen L, Sun J, Bai W, Jiao R, Hu Y, Peng X, He Y, Ou S. Cytoprotective mechanism of ferulic acid against high glucose-induced oxidative stress in cardiomyocytes and hepatocytes. fnr [Internet]. 2016Feb.10 [cited 2019Nov.21];600. Available from: https://foodandnutritionresearch.net/index.php/fnr/article/view/1046
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Original Articles