Antiobesity and anti-inflammation effects of Hakka stir-fried tea of different storage years on high-fat diet-induced obese mice model via activating the AMPK/ACC/CPT1 pathway

  • Qiuhua Li Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Xingfei Lai Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Lingli Sun Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Junxi Cao Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Caijin Ling Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Wenji Zhang Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Limin Xiang Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Ruohong Chen Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
  • Dongli Li School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China
  • Shili Sun Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China
DOI: https://doi.org/10.29219/fnr.v64.1681
Keywords: Hakka stir-fried tea; obesity; inflammation; fat accumulation; hepatic steatosis; AMPK/ACC/CPT1 pathway

Abstract

Background: As a typical representative of metabolic syndrome, obesity is also one of the extremely dangerous factors of cardiovascular diseases. Thus, the prevention and treatment of obesity has gradually become a global campaign. There have been many reports that green tea is effective in preventing obesity, but as a kind of green tea with regional characteristics, there have been no reports that Hakka stir-fried tea (HT) of different storage years has a weight loss effect.

Aims: The aim was to investigate the effect of HT in diet-induced obese mice.

Methods: The mice were divided into five groups as follows: the control group received normal diet; the obese model group received high-fat diet; and HT2003, HT2008, and HT2015 groups, after the induction of obesity via a high-fat diet, received HT of different storage years treatment for 6 weeks, respectively.

Results: It was observed that HT decreased the levels of serum and liver triglyceride; the ratio of liver to body weight; accumulation of epididymal, perirenal, and mesenteric fat; the degree of hepatic steatosis; and adipocyte hypertrophy, with the concomitant reduction of body weight. Moreover, HT decreased the expression levels of proinflammatory cytokines tumor necrosis factor α (TNF α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and reduced fatty acid synthase (FAS) activity in liver tissue of obese mice. In addition, HT treatment also increased the phosphorylation of AMP-activated protein kinase (AMPK) and its direct downstream proteins, acetyl coenzyme A carboxylase (ACC), and carnitine palmitoyltransferase I (CPT-1), which participate in FAS pathway.

Conclusions: These findings demonstrate that HT treatment has a potential protection on high-fat diet-induced obesity mice via activating the AMPK/ACC/CPT1 pathway, and to a certain extent, it has nothing to do with the storage time of three kinds of HT..

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References


  1. Hamer M, Stamatakis E. Metabolically healthy obesity and risk of all-cause and cardiovascular disease mortality. J Clin Endocrinol Metab 2012; 97: 2482–8. doi: 10.1210/jc.2011-3475

  2. Galassi A, Reynolds K, He J. Metabolic syndrome and risk of cardiovascular disease: a meta-analysis. Am J Med 2006; 119: 812–9. doi: 10.1016/j.amjmed.2006.02.031

  3. Alexander CM, Landsman PB, Grundy SM. Metabolic syndrome and hyperglycemia: congruence and divergence. Am J Cardiol 2006; 98: 982–5. doi: 10.1016/j.amjcard.2006.04.046

  4. Rinkuniene E, Butkute E, Puronaite R, Petrulioniene Z, Dzenkeviciute V, Kasiulevicius V, et al. Arterial function parameters in patients with metabolic syndrome and severe hypertriglyceridemia. J Clin Lipidol 2017; 11: 901–7. doi: 10.1016/j.jacl.2017.04.119

  5. Khitan Z, Kim DH. Fructose: a key factor in the development of metabolic syndrome and hypertension. J Nutr Metab 2013; 2013: 682673. doi: 10.1155/2013/682673

  6. Crombie IK, Irvine L, Elliott L, Wallace H. Targets to tackle the obesity epidemic: a review of twelve developed countries. Public Health Nutr 2009; 12: 406–13. doi: 10.1017/S1368980008002292

  7. Wang Y, Wang L, Qu W. New national data show alarming increase in obesity and noncommunicable chronic diseases in China. Eur J Clin Nutr 2017; 71: 149–50. doi: 10.1038/ejcn.2016.171

  8. Han LL, Wang YX, Li J, Zhang XL, Bian C, Wang H, et al. Gender differences in associations of serum ferritin and diabetes, metabolic syndrome, and obesity in the China Health and Nutrition Survey. Mol Nutr Food Res 2014; 58: 2189–95. doi: 10.1002/mnfr.201400088

  9. Finkelstein EA, Ruhm CJ, Kosa KM. Economic causes and consequences of obesity. Annu Rev Public Health 2005; 26: 239–57. doi: 10.1146/annurev.publhealth.26.021304.144628

  10. Cali AM, Caprio S. Ectopic fat deposition and the metabolic syndrome in obese children and adolescents. Horm Res 2009; 71 Suppl 1: 2–7. doi: 10.1159/000178028

  11. Padwal RS, Majumdar SR. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet 2007; 369: 71–7. doi: 10.1016/S0140-6736(07)60033-6

  12. Chacko SM, Thambi PT, Kuttan R, Nishigaki I. Beneficial effects of green tea: a literature review. Chin Med 2010; 5: 13. doi: 10.1186/1749-8546-5-13

  13. Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea – a review. J Am Coll Nutr 2006; 25: 79–99. doi: 10.1080/07315724.2006.10719518

  14. Li J, Hua J, Zhou Q, Dong C, Wang J, Deng Y, et al. Comprehensive lipidome-wide profiling reveals dynamic changes of tea lipids during manufacturing process of black tea. J Agric Food Chem 2017; 65: 10131–40. doi: 10.1021/acs.jafc.7b03875

  15. Sae-tan S, Grove KA, Lambert JD. Weight control and prevention of metabolic syndrome by green tea. Pharmacol Res 2011; 64: 146–54. doi: 10.1016/j.phrs.2010.12.013

  16. Valcic S, Muders A, Jacobsen NE, Liebler DC, Timmermann BN. Antioxidant chemistry of green tea catechins. Identification of products of the reaction of (-)-epigallocatechin gallate with peroxyl radicals. Chem Res Toxicol 1999; 12: 382–6. doi: 10.1021/tx990003t

  17. Xu YQ, Ji WB, Yu P, Chen JX, Wang F, Yin JF. Effect of extraction methods on the chemical components and taste quality of green tea extract. Food Chem 2018; 248: 146–54. doi: 10.1016/j.foodchem.2017.12.060

  18. Tachibana H, Koga K, Fujimura Y, Yamada K. A receptor for green tea polyphenol EGCG. Nat Struct Mol Biol 2004; 11: 380–1. doi: 10.1038/nsmb743

  19. Kuriyama S. Green tea consumption and prevention of coronary artery disease. Circ J 2010; 74: 248–9. doi: 10.1253/circj.CJ-09-1004

  20. Mattioli AV. Effects of caffeine and coffee consumption on cardiovascular disease and risk factors. Future Cardiol 2007; 3: 203–12. doi: 10.2217/14796678.3.2.203

  21. Lee S, Hudson R, Kilpatrick K, Graham TE, Ross R. Caffeine ingestion is associated with reductions in glucose uptake independent of obesity and type 2 diabetes before and after exercise training. Diabetes Care 2005; 28: 566–72. doi: 10.2337/diacare.28.3.566

  22. Rains TM, Agarwal S, Maki KC. Antiobesity effects of green tea catechins: a mechanistic review. J Nutr Biochem 2011; 22: 1–7. doi: 10.1016/j.jnutbio.2010.06.006

  23. Ashida H, Furuyashiki T, Nagayasu H, Bessho H, Sakakibara H, Hashimoto T, et al. Anti-obesity actions of green tea: possible involvements in modulation of the glucose uptake system and suppression of the adipogenesis-related transcription factors. Biofactors 2004; 22: 135–40. doi: 10.1002/biof.5520220126

  24. Suzuki T, Pervin M, Goto S, Isemura M, Nakamura Y. Beneficial effects of tea and the green tea catechin epigallocatechin-3-gallate on obesity. Molecules 2016; 21: 1305. doi: 10.3390/molecules21101305

  25. Furuyashiki T, Nagayasu H, Aoki Y, Bessho H, Hashimoto T, Kanazawa K, et al. Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARgamma2 and C/EBPalpha in 3T3-L1 cells. Biosci Biotechnol Biochem 2004; 68: 2353–9. doi: 10.1271/bbb.68.2353

  26. Chen KH, Li PC, Lin WH, Chien CT, Low BH. Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver. Biosci Biotechnol Biochem 2011; 75: 1668–76. doi: 10.1271/bbb.110163

  27. Nam M, Choi MS, Choi JY, Kim N, Kim MS, Jung S, et al. Effect of green tea on hepatic lipid metabolism in mice fed a high-fat diet. J Nutr Biochem 2018; 51: 1–7. doi: 10.1016/j.jnutbio.2017.09.002

  28. Choo JJ. Green tea reduces body fat accretion caused by high-fat diet in rats through beta-adrenoceptor activation of thermogenesis in brown adipose tissue. J Nutr Biochem 2003; 14: 671–6. doi: 10.1016/j.jnutbio.2003.08.005

  29. Nagao T, Komine Y, Soga S, Meguro S, Hase T, Tanaka Y, et al. Ingestion of a tea rich in catechins leads to a reduction in body fat and malondialdehyde-modified LDL in men. Am J Clin Nutr 2005; 81: 122–9. doi: 10.1093/ajcn/81.1.122

  30. Wolfram S, Wang Y, Thielecke F. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res 2006; 50: 176–87. doi: 10.1002/mnfr.200500102

  31. Lin JK, Lin-Shiau SY. Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols. Mol Nutr Food Res 2006; 50: 211–7. doi: 10.1002/mnfr.200500138

  32. Basu A, Sanchez K, Leyva MJ, Wu M, Betts NM, Aston CE, et al. Green tea supplementation affects body weight, lipids, and lipid peroxidation in obese subjects with metabolic syndrome. J Am Coll Nutr 2010; 29: 31–40. doi: 10.1080/07315724.2010.10719814

  33. Singh BN, Shankar S, Srivastava RK. Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol 2011; 82: 1807–21. doi: 10.1016/j.bcp.2011.07.093

  34. Huang J, Wang Y, Xie Z, Zhou Y, Zhang Y, Wan X. The anti-obesity effects of green tea in human intervention and basic molecular studies. Eur J Clin Nutr 2014; 68: 1075–87. doi: 10.1038/ejcn.2014.143

  35. Neyrinck AM, Bindels LB, Geurts L, Van Hul M, Cani PD, Delzenne NM. A polyphenolic extract from green tea leaves activates fat browning in high-fat-diet-induced obese mice. J Nutr Biochem 2017; 49: 15–21. doi: 10.1016/j.jnutbio.2017.07.008

  36. Hsu CH, Tsai TH, Kao YH, Hwang KC, Tseng TY, Chou P. Effect of green tea extract on obese women: a randomized, double-blind, placebo-controlled clinical trial. Clin Nutr 2008; 27: 363–70. doi: 10.1016/j.clnu.2008.03.007

  37. Park EJ, Lee JH, Yu GY, He G, Ali SR, Holzer RG, et al. Dietary and genetic obesity promote liver inflammation and tumorigenesis by enhancing IL-6 and TNF expression. Cell 2010; 140: 197–208. doi: 10.1016/j.cell.2009.12.052

  38. Cunha CA, Lira FS, Rosa Neto JC, Pimentel GD, Souza GI, da Silva CM, et al. Green tea extract supplementation induces the lipolytic pathway, attenuates obesity, and reduces low-grade inflammation in mice fed a high-fat diet. Mediators Inflamm 2013; 2013: 635470. doi: 10.1155/2013/635470

  39. Li J, Sasaki GY, Dey P, Chitchumroonchokchai C, Labyk AN, McDonald JD, et al. Green tea extract protects against hepatic NFkappaB activation along the gut-liver axis in diet-induced obese mice with nonalcoholic steatohepatitis by reducing endotoxin and TLR4/MyD88 signaling. J Nutr Biochem 2018; 53: 58–65. doi: 10.1016/j.jnutbio.2017.10.016

  40. Kim CS, Lee SC, Kim YM, Kim BS, Choi HS, Kawada T, et al. Visceral fat accumulation induced by a high-fat diet causes the atrophy of mesenteric lymph nodes in obese mice. Obesity (Silver Spring) 2008; 16: 1261–9. doi: 10.1038/oby.2008.55

  41. Basu A, Du M, Sanchez K, Leyva MJ, Betts NM, Blevins S, et al Green tea minimally affects biomarkers of inflammation in obese subjects with metabolic syndrome. Nutrition 2011; 27: 206–13. doi: 10.1016/j.nut.2010.01.015

  42. Stote KS, Clevidence BA, Novotny JA, Henderson T, Radecki SV, Baer DJ. Effect of cocoa and green tea on biomarkers of glucose regulation, oxidative stress, inflammation and hemostasis in obese adults at risk for insulin resistance. Eur J Clin Nutr 2012; 66: 1153–9. doi: 10.1038/ejcn.2012.101

  43. Uchiyama S, Taniguchi Y, Saka A, Yoshida A, Yajima H. Prevention of diet-induced obesity by dietary black tea polyphenols extract in vitro and in vivo. Nutrition 2011; 27: 287–92. doi: 10.1016/j.nut.2010.01.019

  44. Ramadan G, El-Beih NM, Abd El-Ghffar EA. Modulatory effects of black v. green tea aqueous extract on hyperglycaemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models. Br J Nutr 2009; 102: 1611. doi: 10.1017/s000711450999208x

  45. Park HJ, Dinatale DA, Chung MY, Park YK, Lee JY, Koo SI, et al. Green tea extract attenuates hepatic steatosis by decreasing adipose lipogenesis and enhancing hepatic antioxidant defenses in ob/ob mice. J Nutr Biochem 2011; 22: 393–400. doi: 10.1016/j.jnutbio.2010.03.009

  46. Chen YQ, Zhang W, Chen Q, Dong FJ, Liu XH, Gan DP, et al. Effect of Hubei green brick tea on weight reducing. J Tea Sci 2008; 8: 363–9. doi: 10.13305/j.cnki.jts.2008.05.011

  47. Bose M, Lambert JD, Ju J, Reuhl KR, Shapses SA, Yang CS. The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice. J Nutr 2008; 138: 1677–83. doi: 10.1089/jmf.2007.0500

  48. Cheng T, Peng CX, Gong JS, Wang QP, Zhou HJ. Effect of theabrownin extracted from Pu-erh tea on the metabolism of blood lipid in hyperlipidmia rats. Chin J Food Sci 2011; 1: 20–27. doi: 10.16429/j.1009-7848.2011.01.032

  49. Kim H, Hiraishi A, Tsuchiya K, Sakamoto K. (-) Epigallocatechin gallate suppresses the differentiation of 3T3-L1 preadipocytes through transcription factors FoxO1 and SREBP1c. Cytotechnology 2010; 62: 245–55. doi: 10.1007/s10616-010-9285-x

  50. Unno K, Yamamoto H, Maeda K, Takabayashi F, Yoshida H, Kikunaga N, et al. Protection of brain and pancreas from high-fat diet: effects of catechin and caffeine. Physiol Behav 2009; 96: 262–9. doi: 10.1016/j.physbeh.2008.10.009

  51. Yi GC, Yao LH, Guo YL. Effect of fermentation methods on black tea quality. Fujian Tea 2019; 41: 7–8.

  52. Yang XQ, Liu YQ, Liu Y, Luo J. Comparative analysis of tea polyphenols and catechins in green tea and black tea of Fuding Dabai tea by HPLC. South China Agric 2019; 13: 22–7. doi: 10.19415/j.cnki.1673-890x.2019.28.006

  53. Rocha A, Bolin AP, Cardoso CA, Otton R. Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity. Eur J Nutr 2016; 55: 2231–44. doi: 10.1007/s00394-015-1033-8

  54. Niu Y, Yuan H, Fu L. Aerobic exercise’s reversal of insulin resistance by activating AMPKalpha-ACC-CPT1 signaling in the skeletal muscle of C57BL/6 mice. Int J Sport Nutr Exerc Metab 2010; 20: 370–80. doi: 10.1123/ijsnem.20.5.370

  55. He Z, Peng Y, Duan W, Tian Y, Zhang J, Hu T, et al Aspirin regulates hepatocellular lipid metabolism by activating AMPK signaling pathway. J Toxicol Sci 2015; 40: 127–36. doi: 10.2131/jts.40.127

  56. Abdel-Magid AF. Fatty Acid Synthase (FASN) inhibitors as potential treatment for cancer, obesity, and liver related disorders. ACS Med Chem Lett 2015; 6: 838–9. doi: 10.1021/acsmedchemlett.5b00275

  57. Kamisoyama H, Honda K, Tominaga Y, Yokota S, Hasegawa S. Investigation of the anti-obesity action of licorice flavonoid oil in diet-induced obese rats. Biosci Biotechnol Biochem 2008; 72: 3225–31. doi: 10.1271/bbb.80469

  58. Yang DJ, Chang YY, Hsu CL, Liu CW, Lin YL, Lin YH, et al. Antiobesity and hypolipidemic effects of polyphenol-rich longan (Dimocarpus longans Lour.) flower water extract in hypercaloric-dietary rats. J Agric Food Chem 2010; 58: 2020–7. doi: 10.1021/jf903355q

Published
2020-06-08
How to Cite
Li Q., Lai X., Sun L., Cao J., Ling C., Zhang W., Xiang L., Chen R., Li D., & Sun S. (2020). Antiobesity and anti-inflammation effects of Hakka stir-fried tea of different storage years on high-fat diet-induced obese mice model via activating the AMPK/ACC/CPT1 pathway. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.1681
Issue
Vol 64 (2020)
Section
Original Articles

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