Antidiabetic effects of a lipophilic extract obtained from flowers of Wisteria sinensis by activating Akt/GLUT4 and Akt/GSK3β

  • Yibing Lv School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China https://orcid.org/0000-0002-8768-4489
  • Wenjie Ren School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Yirui Zhang School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Yun Huang School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Ji Hao School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Kun Ma School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Yuanren Ma School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
  • Xinzhou Yang School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
Keywords: Antidiabetes, GLUT4, dyslipidemia, hyperglycemia, insulin resistance

Abstract

Background: Type 2 diabetes mellitus is primarily caused by insulin resistance (IR) in insulin-sensitive tissues, including liver, white adipose tissues (WAT), and skeletal muscles. Discovering nutritious foods with antidiabetic effects is of great significance. Numerous published reports indicated that protein kinase B (Akt) and glucose transporter 4 (GLUT4) play crucial roles in ameliorating IR and diabetic symptoms.

Objective: In the present study, antidiabetic effects and the potential mechanism of action of WS-PE (a lipophilic extract from edible flowers of Wisteria sinensis) were explored with L6 cells in vitro and in high-fat diet (HFD) + Streptozocin (STZ)-induced diabetic mice in vivo.

Design: In vivo, HFD + STZ-induced diabetic mice were used as diabetic models to investigate the potential antidiabetic and antidyslipidemic activities. In vitro, a novel GLUT4 translocation assay system was established to evaluate the potential effects of WS-PE on GLUT4 translocation. Western blot analysis was adopted to investigate the molecular mechanisms of WS-PE both in vivo and in vitro.

Results: In vitro, WS-PE increased glucose uptake by stimulating GLUT4 expression and translocation, which were regulated by Akt phosphorylation. In vivo, the WS-PE treatment ameliorated the hyperglycemia, IR, and dyslipidemia and reversed hepatic steatosis and pancreatic damage in diabetic mice. The WS-PE treatment increased GLUT4 expression by Akt activation in WAT and skeletal muscle. Akt activation stimulated GSK3β phosphorylation in liver and skeletal muscles, indicating that WS-PE showed regulatory effects on glycogen synthesis in liver and skeletal muscles.

Conclusion: These in vitro and in vivo results indicated that the WS-PE treatment exerted antidiabetic effects by activating Akt/GLUT4 and Akt/GSK3β.

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Published
2020-09-07
How to Cite
Lv, Y., Ren, W., Zhang, Y., Huang, Y., Hao, J., Ma, K., Ma, Y., & Yang, X. (2020). Antidiabetic effects of a lipophilic extract obtained from flowers of <em>Wisteria sinensis</em&gt; by activating Akt/GLUT4 and Akt/GSK3β. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.3589
Section
Original Articles