Antioxidative and antidiabetic effects of germinated rough rice extract in 3T3-L1 adipocytes and C57BLKS/J-db/db mice

  • Heon Sang Jeong Chungbuk National University
  • Youn Ri Lee Department of Food and Nutrition, Daejeon Health Sciences College
  • Sang Hoon Lee Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration
  • Gwi Yeong Jang Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration
  • Yoon Jeong Lee Department of Food Science and Biotechnology, Chungbuk National University
  • Min Young Kim Department of Food Science and Biotechnology, Chungbuk National University
  • Yun-Bae Kim College of Veterinary Medicine, Chungbuk National University
  • Junsoo Lee Department of Food Science and Biotechnology, Chungbuk National University
Keywords: germinated rough rice, antioxidant property, type II diabetes, glucose uptake, C57BLKS/J-db/db mice


Background: To overcome disadvantages of germinated brown rice, we germinated rough rice and tested effects of its useful ingredients on prevention of diabetes.

Objective: This study investigated the in vitro antioxidant and in vivo antidiabetic effects of rough rice (Oryza sativa L.) with hulls, before and after germination. Rough rice was germinated for 4 days and extracted with water at 120°C.

Design: This study measured antioxidants and antioxidative effects and inhibitory activities against α-amylase and α-glucosidase of rough rice before and after germination and investigated antidiabetic effects of rough rice through animal experiments.

Results: All these factors increased after germination. Also, α-amylase and α-glucosidase inhibition and glucose uptake by 3T3-L1 adipocytes were significantly increased after germination. Oral administration of the germinated rough rice extract for 8 weeks significantly increased insulin levels and decreased blood glucose levels in a C57BLKS/J-db/db mice model. Immunohistochemical analysis showed that germinated rough rice effectively protected against liver, kidney, and pancreatic tissue damage.

Discussion: Useful ingredients in germinated rough rice could be used to prevent diabetes.

Conclusions: These results suggest that germinated rough rice extract had a beneficial effect on diabetes by increasing the antioxidant activity and further purification studies are necessary to elucidate the mechanism of the extract’s antidiabetic activity.


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How to Cite
Jeong HS, Lee YR, Lee SH, Jang GY, Lee YJ, Kim MY, Kim Y-B, Lee J. Antioxidative and antidiabetic effects of germinated rough rice extract in 3T3-L1 adipocytes and C57BLKS/J-<em>db/db</em&gt; mice. fnr [Internet]. 2019Nov.28 [cited 2019Dec.6];630. Available from:
Original Articles