Nostoc sphaeroids Kütz powder ameliorates diet-induced hyperlipidemia in C57BL/6j mice
Background: Hypercholesterolemia is a disease associated with numerous health problems. Growing evidence indicates that hypercholesterolemia, hyperlipidemia is closely linked to chronic inflammation, which can lead to cardiovascular disease, fatty liver disease, and type 2 diabetes.
Objective: The purpose of this study was to investigate the protective effect of Nostoc sphaeroids Kütz (NO) on diet-induced hyperlipidemia in mice.
Design: At first, experimental animals received a high-fat diet (HFD) for 4 weeks, and then received a HFD supplemented with 2.5% or 7.5% NO for 6 weeks. In the current study, results show that treatment with NO decreases weight gain and liver index induced by HFD. In addition, the serum levels of TC, TG and LDL are significantly decreased in NO treatment groups.
Results: From the results of Oil Red staining and Hematoxylin and eosin staining (HE), treatment with NO significantly reduces liver lipid accumulation and protect liver structure. Further analysis reveals that NO has positive effects on liver lipid metabolism and inflammation, as showed by the lower protein expressions of FAS and SREBP-1, the lower concentrations of TNF-α, IL-1β, IL-6, and the lower gene expressions of TNF-α, IL-1β, IL-6 and NF-kB.
Conclusions: Our results indicate that NO may significantly ameliorate diet-induced hyperlipidemia, which is possibly associated with improving liver lipid metabolism and reducing chronic inflammation.
- Lee KS, Chun SY, Kwon YS, Kim S, Nam KS. Deep sea water improves hypercholesterolemia and hepatic lipid accumulation through the regulation of hepatic lipid metabolic gene expression. Mol Med Rep 2017; 15(5): 2814–22. doi: 10.3892/mmr.2017.6317
- Hoyt CL, Burnette JL, Auster-Gussman L. ‘Obesity is a disease’: examining the self-regulatory impact of this public-health message. Psychol Sci 2014; 25(4): 997–1002. doi: 10.1177/0956797613516981
- Osborn O, Olefsky JM. The cellular and signaling networks linking the immune system and metabolism in disease. Nat Med 2012; 18(3): 363–74. doi: 10.1038/nm.2627
- Feuerer M, Herrero L, Cipolletta D, Naaz A, Wong J, Nayer A, et al. Lean, but not obese, fat is enriched for a unique population of regulatory T cells that affect metabolic parameters. Nat Med 2009; 15(8): 930–9. doi: 10.1038/nm.2002
- Bin-Jumah MN. Monolluma quadrangula protects against oxidative stress and modulates LDL receptor and fatty acid synthase gene expression in hypercholesterolemic rats. Oxid Med Cell Longev 2018; 2018: 3914384. doi: 10.1155/2018/3914384
- Chun YS, Ku SK, Kim JK, Park S, Cho IH, Lee NJ. Hepatoprotective and anti-obesity effects of Korean blue honeysuckle extracts in high fat diet-fed mice. J Exerc Nutrition Biochem 2018; 22(4): 39–54. doi: 10.20463/jenb.2018.0029
- Calpe-Berdiel L, Escola-Gil JC, Ribas V, Navarro-Sastre A, Garces-Garces J, Blanco-Vaca F. Changes in intestinal and liver global gene expression in response to a phytosterol-enriched diet. Atherosclerosis 2005; 181(1): 75–85. doi: 10.1016/j.atherosclerosis.2004.11.025
- Chan YK, Brar MS, Kirjavainen PV, Chen Y, Peng J, Li D, et al. High fat diet induced atherosclerosis is accompanied with low colonic bacterial diversity and altered abundances that correlates with plaque size, plasma A-FABP and cholesterol: a pilot study of high fat diet and its intervention with Lactobacillus rhamnosus GG (LGG) or telmisartan in ApoE(-/-) mice. BMC Microbiol 2016; 16(1): 264. doi: 10.1186/s12866-016-0883-4
- Dai J, Liang K, Zhao S, Jia W, Liu Y, Wu H, et al. Chemoproteomics reveals baicalin activates hepatic CPT1 to ameliorate diet-induced obesity and hepatic steatosis. Proc Natl Acad Sci U S A 2018; 115(26): E5896–E905. doi: 10.1073/pnas.1801745115
- He K, Li X, Xiao Y, Yong Y, Zhang Z, Li S, et al. Hypolipidemic effects of Myrica rubra extracts and main compounds in C57BL/6j mice. Food Funct 2016; 7(8): 3505–15. doi: 10.1039/c6fo00623j
- Shimizu T, Mori K, Ouchi K, Kushida M, Tsuduki T. Effects of dietary intake of Japanese mushrooms on visceral fat accumulation and gut microbiota in mice. Nutrients 2018; 10(5): 1–16. doi: 10.3390/nu10050610
- Johnson HE, King SR, Banack SA, Webster C, Callanaupa WJ, Cox PA. Cyanobacteria (Nostoc commune) used as a dietary item in the Peruvian highlands produce the neurotoxic amino acid BMAA. J Ethnopharmacol 2008; 118(1): 159–65. doi: 10.1016/j.jep.2008.04.008
- Chen Z, Juneau P, Qiu B. Effects of three pesticides on the growth, photosynthesis and photoinhibition of the edible cyanobacterium Ge-Xian-Mi (Nostoc). Aquat Toxicol 2007; 81(3): 256–65. doi: 10.1016/j.aquatox.2006.12.008
- Hao Z, Li D, Li Y, Wang Z, Xiao Y, Wang G, et al. Nostoc sphaeroides Kützing, an excellent candidate producer for CELSS. Adv Space Res 2011; 48(10): 1565–71. doi: 10.1016/j.asr.2011.06.035
- Yang Y, Park Y, Cassada DA, Snow DD, Rogers DG, Lee J. In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kutzing and Spirulina plantensis. Food Chem Toxicol 2011; 49(7): 1560–4. doi: 10.1016/j.fct.2011.03.052
- Ku CS, Pham TX, Park Y, Kim B, Shin MS, Kang I, et al. Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-kappaB pathway in macrophages and splenocytes. Biochim Biophys Acta 2013; 1830(4): 2981–8. doi: 10.1016/j.bbagen.2013.01.018
- Ku CS, Kim B, Pham TX, Yang Y, Wegner CJ, Park YK, et al. Blue-Green Algae inhibit the development of atherosclerotic lesions in apolipoprotein E knockout mice. J Med Food 2015; 18(12): 1299–306. doi: 10.1089/jmf.2015.0025
- Ku CS, Kim B, Pham TX, Yang Y, Weller CL, Carr TP, et al. Hypolipidemic effect of a blue-green alga (nostoc commune) is attributed to its nonlipid fraction by decreasing intestinal cholesterol absorption in C57BL/6J mice. J Med Food 2015; 18(11): 1214–22. doi: 10.1089/jmf.2014.0121
- Kang HJ, Pichiah PBT, Abinaya RV, Sohn HS, Cha YS. Hypocholesterolemic effect of quercetin-rich onion peel extract in C57BL/6J mice fed with high cholesterol diet. Food Sci Biotechnol 2016; 25(3): 855–60. doi: 10.1007/s10068-016-0141-4
- Lei YF, Chen JL, Wei H, Xiong CM, Zhang YH, Ruan JL. Hypolipidemic and anti-inflammatory properties of Abacopterin A from Abacopteris penangiana in high-fat diet-induced hyperlipidemia mice. Food Chem Toxicol 2011; 49(12): 3206–10. doi: 10.1016/j.fct.2011.08.027
- Chang CJ, Lin CS, Lu CC, Martel J, Ko YF, Ojcius DM, et al. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun 2015; 6: 7489. doi: 10.1038/ncomms8489
- Um MY, Moon MK, Ahn J, Youl Ha T. Coumarin attenuates hepatic steatosis by down-regulating lipogenic gene expression in mice fed a high-fat diet. Br J Nutr 2013; 109(9): 1590–7. doi: 10.1017/S0007114512005260
- Kim YJ, Houng SJ, Kim JH, Kim YR, Ji HG, Lee SJ. Nanoemulsified green tea extract shows improved hypocholesterolemic effects in C57BL/6 mice. J Nutr Biochem 2012; 23(2): 186–91. doi: 10.1016/j.jnutbio.2010.11.015
- Shigematsu K, Watanabe Y, Nakano H, Kyoto Stroke Registry C. Influences of hyperlipidemia history on stroke outcome; a retrospective cohort study based on the Kyoto Stroke Registry. BMC Neurol 2015; 15: 44. doi: 10.1186/s12883-015-0297-1
- Zhai X, Lin D, Zhao Y, Li W, Yang X. Effects of dietary fiber supplementation on fatty acid metabolism and intestinal microbiota diversity in C57BL/6J mice fed with a high-fat diet. J Agric Food Chem 2018; 66(48): 12706–18. doi: 10.1021/acs.jafc.8b05036
- Zhang S, Shan JJ, Zhu Y, Frenkel AI, Patlolla A, Huang W, et al. WGS catalysis and in situ studies of CoO(1-x), PtCo(n)/Co3O4, and Pt(m)Co(m')/CoO(1-x) nanorod catalysts. J Am Chem Soc 2013; 135(22): 8283-93. doi: 10.1021/ja401967y
- Hou Y, Lohe MR, Zhang J, Liu S, Zhuang X, Feng X. Vertically oriented cobalt selenide/NiFe layered-double-hydroxide nanosheets supported on exfoliated graphene foil: an efficient 3D electrode for overall water splitting. Energy Environ Sci 2016; 9(2): 478–83. doi: 10.1039/c5ee03440j
- Horton JD, Goldstein JL, Brown MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Investig 2002; 109(9): 1125–31. doi: 10.1172/jci0215593
- Lubbad A, Oriowo MA, Khan I. Curcumin attenuates inflammation through inhibition of TLR-4 receptor in experimental colitis. Mol Cell Biochem 2009; 322(1–2): 127–35. doi: 10.1007/s11010-008-9949-4
- Qiu L, List EO, Kopchick JJ. Differentially expressed proteins in the pancreas of diet-induced diabetic mice. Mol Cell Proteomics 2005; 4(9): 1311–18. doi: 10.1074/mcp.M500016-MCP200
- Soares A, Beraldi EJ, Ferreira PE, Bazotte RB, Buttow NC. Intestinal and neuronal myenteric adaptations in the small intestine induced by a high-fat diet in mice. BMC Gastroenterol 2015; 15: 3. doi: 10.1186/s12876-015-0228-z
- Flegal KM, Graubard BI, Williamson DF, Gail MH. Cause-specific excess deaths associated with underweight, overweight, and obesity. JAMA 2007; 298(17): 2028–37. doi: 10.1001/jama.298.17.2028
- Nam M, Choi MS, Choi JY, Kim N, Kim MS, Jung S, et al. Effect of green tea on hepatic lipid metabolism in mice fed a high-fat diet. J Nutr Biochem 2018; 51: 1–7. doi: 10.1016/j.jnutbio.2017.09.002
- Kang C, Wang B, Kaliannan K, Wang X, Lang H, Hui S, et al. Gut microbiota mediates the protective effects of dietary capsaicin against chronic low-grade inflammation and associated obesity induced by high-fat diet. MBio 2017; 8(3): 1–14. doi: 10.1128/mBio.00470-17
- Prakash S, Rai U, Kosuru R, Tiwari V, Singh S. Amelioration of diet-induced metabolic syndrome and fatty liver with Sitagliptin via regulation of adipose tissue inflammation and hepatic Adiponectin/AMPK levels in mice. Biochimie 2019. doi: 10.1016/j.biochi.2019.11.005
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