A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

  • Xiaoqing Xu
  • Qing Peng
  • Yuwei Zhang
  • Dandan Tian
  • Pengbo Zhang
  • Ying Huang
  • Lan Ma
  • Yu Qiao
  • Bo Shi
Keywords: Lactobacillus coryniformis NA-3; Exopolysaccharide; Antioxidant; Anti-biofilm; Inhibition; Dispersion

Abstract

Background: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 isolated from northeast Chinese sauerkraut (Suan Cai).

Methods: Lactobacillus coryniformis NA-3 was identified with 16S rDNA amplification and Neighbor Joining (NJ) phylogenetic analysis. EPS derived from Lactobacillus coryniformis NA-3 (EPS-NA3) was analyzed, including compositions by high-performance liquid chromatography (HPLC), functional groups by Fourier-transform infrared spectroscopy (FT-IR) and glycosidic bond configuration by Hydrogen-1 Nuclear Magnetic Resonance (1H NMR). Antioxidant activity of EPS was evaluated with hydroxyl and superoxide radical-scavenging. Anti-biofilm activities of EPS-NA3 were checked through inhibition and dispersion.

Results: The monosaccharide composition of EPS included α-rhamnose, α-mannose, α-galactose, and α-glucose in a ratio of 2.6:1.0:5.0:3.3. The free radical-scavenging abilities of EPS-NA3 were 37.77% ± 1.56% and 78.87% ± 3.07% on hydroxyl and superoxide reactive oxygen species respectively. Moreover, EPS-NA3 attenuated the formation of Bacillus cereus and Salmonella typhimurium biofilms by inhibition ratios of approximately 80% and 40% respectively. Additionally, treatment with EPS-NA3 dispersed established biofilms of B. cereus and S. typhimurium by approximately 90% and 20% respectively.

Conclusion: These results suggest that EPS-NA3 may be developed as antioxidant and anti-biofilm agents for industrial and clinical applications due to its capacity of scavenging free radicals, inhibition of bacterial biofilm formation, and dispersion of established biofilms.

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Published
2020-04-03
How to Cite
Xu, X., Peng, Q., Zhang, Y., Tian, D., Zhang, P., Huang, Y., Ma, L., Qiao, Y., & Shi, B. (2020). A novel exopolysaccharide produced by <em>Lactobacillus coryniformis</em> NA-3 exhibits antioxidant and biofilm-inhibiting properties <em>in vitro</em&gt;. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.3744
Section
Original Articles