Effects of fermented red bean extract on nephropathy in streptozocin-induced diabetic rats

  • Kung-Chi Chan Department of Food and Nutrition, Providence University, Taichung
  • Kar-Eng Kok Department of Food and Nutrition, Providence University, Taichung
  • Keh-Feng Huang Department of Applied Chemistry, Providence University, Taichung
  • Yao-Lin Weng Department of Food and Nutrition, Providence University, Taichung
  • Yun-Chin Chung Department of Food and Nutrition, Providence University, Taichun
DOI: https://doi.org/10.29219/fnr.v64.4272
Keywords: advanced-glycation end-products, C-reactive protein, 24-hour creatinine clearance, diabetic rats, natto-red bean extract

Abstract

Background: The antioxidant effects of Bacillus subtilis-fermented red bean (natto-red bean) extract (NRBE) in young (6 weeks old) Sprague–Dawley rats and aged (12 months old) mice had been reported previously.

Objective: To evaluate the antioxidant and anti-inflammatory effects of NRBE in the kidneys of streptozocin- induced diabetic rats.

Design: Normal control rats and diabetic rats were orally gavaged with saline and low-dose NRBE (100 mg/ kg body weight [BW]), medium-dose NRBE (200 mg/kg BW), and high-dose NRBE (500 mg/kg BW), for 12 weeks and then sacrificed. Concentration of fasting glucose, adiponectin, renal function markers, antioxidative markers, and pro-inflammatory markers were measured.

Results: Oral administration of 50% ethanolic extract of NRBE with a dosage of 100 mg/kg BW, 200 mg/kg BW, or 500 mg/kg BW could improve the symptoms of kidney enlargement and renal function. Supplementation of NRBE can effectively inhibit the formation of renal reactive oxygen species and advanced-glycation end-products and increase renal glutathione content and serum adiponectin. A low dose of NRBE (100 mg/ kg BW) decreased fasting blood sugar and renal interleukin (IL)-6 expression. Serum C-reactive protein, renal tumor necrosis factor-α, and monocyte chemoattractant protein-1 concentrations were decreased, and renal superoxide dismutase activity was increased in the medium-dose NRBE group. Twenty-four hour creatinine clearance and urinary albumin excretion also improved by medium-dose NRBE supplementation. In NRBE, total phenols and flavonoids were 6.3 mg gallic acid equivalent/g and 12.02 mg rutin equivalent/g, respectively, and kampherol was the major active antioxidant compound.

Conclusion: This study demonstrated that appropriate amount of NRBE, 200 mg/kg BW in rats, could prevent diabetic nephropathy by improving antioxidant status and inhibiting inflammation in renal tissue.

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Published
2020-12-22
How to Cite
Chan K.-C., Kok K.-E., Huang K.-F., Weng Y.-L., & Chung Y.-C. (2020). Effects of fermented red bean extract on nephropathy in streptozocin-induced diabetic rats. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.4272
Issue
Vol 64 (2020)
Section
Original Articles
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