A novel gene, CaATHB-12, negatively regulates fruit carotenoid content under cold stress in Capsicum annuum

  • Rui-Xing Zhang College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
  • Wen-Chao Zhu Guizhou Institute of Pepper, Guiyang, China
  • Guo-Xin Cheng College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
  • Ya-Nan Yu College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
  • Quan-Hui Li College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
  • Saeed ul Haq College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
  • Fazal Said Department of Agriculture, Abdul Wali Khan University, Mardan, Paksitan
  • Zhen-Hui Gong College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
Keywords: pepper; Carotenoids; CaATHB-12; Cold stress; Transgenic Arabidopsis

Abstract

Background: Carotenoids, the secondary metabolites terpenoids, are the largest factors that form the fruit color. Similar to flavonoids, they are not only safe and natural colorants of fruits but also play a role as stress response biomolecules.

Methods: To study the contribution of the key genes in carotenoids biosynthesis, fruit-color formation, and in response to cold stress, we characterized the key regulatory factor CaATHB-12 from the HD-ZIP I sub-gene family members in pepper.

Results: Cold stress enhanced carotenoid accumulation as compared with the normal condition. CaATHB-12 silencing through virus-induced gene silencing changed the fruit color by regulating the carotenoid contents. CaATHB-12 silencing increased the antioxidant enzyme activities in the fruits of pepper, exposed to cold stress, whereas CaATHB-12 overexpression decreased the activities of antioxidant enzymes in the transgenic Arabidopsis lines, exposed to cold stress, suggesting that CaATHB-12 is involved in the regulation of cold stress in the pepper fruits.

Conclusion: Our research will provide insights into the formation of fruit color in pepper and contribution of CaATHB-12 in response to cold stress. Further study should be focused on the interaction between CaATHB-12 and its target gene.

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References


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Published
2020-12-28
How to Cite
Zhang, R.-X., Zhu, W.-C., Cheng, G.-X., Yu, Y.-N., Li, Q.-H., ul Haq, S., Said, F., & Gong, Z.-H. (2020). A novel gene, <em>CaATHB-12</em>, negatively regulates fruit carotenoid content under cold stress in <em>Capsicum annuum</em&gt;. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.3729
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Original Articles