Effect of dietary oils from various sources on carbohydrate and fat metabolism in mice

  • Anna Altberg Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
  • Ran Hovav Department of Field and Vegetable Crops, Plant Sciences Institute, ARO (Volcani Center), Bet Dagan, Israel
  • Nava Chapnik Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
  • Zecharia Madar Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Keywords: oleic acid, soybean oil, gene expression, Hanoch, D7, Triglycerides


Background: Dietary oils differ in their fatty acid composition and the presence of additional microcomponents (antioxidants, etc.). These differences are thought to invoke different biochemical pathways, thus affecting fats and carbohydrates metabolism differently. Olive oil (OO) and soybean oil (SO) are common vegetable oils in the local cuisine. Peanuts oils of local varieties are viewed as potential sources of dietary vegetable oils, especially in the food industry.

Objective: We examined the effect of four different dietary vegetable oils on carbohydrate and lipid metabolism in mice. The selected oils were OO, high in oleic acid, extracted from cultivated high oleic acid peanut (C-PO), regular peanut oil (PO), and SO.

Design: In this study, 32 male C57BL/6J mice were randomly divided into four groups (n = 8 in each group) and were fed with four different diets enriched with 4% (w/w) dietary vegetable oils (OO, C-PO, PO, or SO). After 10 weeks, the mice were sacrificed. Western blot was used to examine proteins such as phospho-AMP-activated protein kinase (p-AMPK), ace-tyl-CoA carboxylase (ACC), cluster of differentiation 36 (CD36), and Sirtuin 1 (SIRT1), whereas real-time polymerase chain reaction (PCR) was used to examine the expression of sterol regulatory element-binding protein-1c (SREBP-1C), fatty acid synthase (FAS), glucose-6-phosphatase (G6Pase), and CD36 transcripts.

Results: In mice-fed SO, lipid accumulation was predominately in adipose tissue, accompanied a tendency decrease in insulin sensitivity. Mice-fed OO had lower plasma triglycerides (TG) and increased hepatic CD36 gene expression. The C-PO group presented lower messenger RNA (mRNA) levels in the liver for all examined genes: SREBP-1c, FAS, G6Pase, and CD36. There were no significant differences in weight gain, plasma cholesterol and high-density lipoprotein (HDL) cholesterol levels, hepatic ACC, SIRT1, AMPK, and CD36 protein levels or in liver function among the diets. Discussion: It seems that as long as fat is consumed in moderation, oil types may play a lesser role in the metabolism of healthy individuals.

Conclusion: This finding has the potential to increase flexibility in choosing oil types for consumption.


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  1. Cinti S. The adipose organ at a glance. DMM 2012; 5(5): 588–94. doi: 10.1242/dmm.009662

  2. Laguna-Camacho A. Influence on adiposity and atherogenic lipaemia of fatty meals and snacks in daily life. J Lipids 2017; 2017: n.p. doi: 10.1155/2017/1375342

  3. Herieka M, Erridge C. High-fat meal induced postprandial inflammation. Mol Nutr Food Res 2014; 58(1): 136–46. doi: 10.1002/mnfr.201300104

  4. Hernandez EA, Kahl S, Seelig A, Begovatz P, Irmler M, Kupriyanova Y, et al. Acute dietary fat intake initiates alterations in energy metabolism and insulin resistance. J Clin Investig 2017; 127(2): 695–708. doi: 10.1172/jci89444

  5. Mirmiran P, Amirhamidi Z, Ejtahed HS, Bahadoran Z, Azizi F. Relationship between diet and non-alcoholic fatty liver disease: a review article. Iran J Public Health 2017; 46(8): 1007–17. PMID: 28894701

  6. Moreno-Fernández S, Garcés-Rimón M, Vera G, Astier J, Landrier JF, Miguel M. high fat/high glucose diet induces metabolic syndrome in an experimental rat model. Nutrients 2018; 10(10): 1502. doi: 10.3390/nu10101502

  7. Hsu M-C, Wang M-E, Jiang Y-F, Liu H-C, Chen Y-C, Chiu C-H. Long-term feeding of high-fat plus high-fructose diet induces isolated impaired glucose tolerance and skeletal muscle insulin resistance in miniature pigs. Diabetol Metab Syndr 2017; 9: 81. doi: 10.1186/s13098-017-0281-6

  8. Velázquez KT, Enos RT, Bader JE, Sougiannis AT, Carson MS, Chatzistamou I, et al. Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice. World J Hepatol 2019; 11(8): 619–37. doi: 10.4254/wjh.v11.i8.619

  9. Huang YY, Gusdon AM, Qu S. Nonalcoholic fatty liver disease: molecular pathways and therapeutic strategies. Lipids Health Dis 2013; 12: 171. doi: 10.1186/1476-511X-12-171

  10. Mozaffarian D, Hao T, Rimm EB, Willett WC, Hu FB. Changes in diet and lifestyle and long-term weight gain in women and men. N Engl J Med 2011; 364(25): 2392–404. doi: 10.1056/NEJMoa1014296

  11. Mente A, de Koning L, Shannon HS, Anand SS. A systematic review of the evidence supporting a causal link between dietary factors and coronary heart disease. Archiv Intern Med 2009; 169(7): 659–69. doi: 10.1001/archinternmed.2009.38

  12. Lamping KG, Nuno DW, Coppey LJ, Holmes AJ, Hu S, Oltman CL, et al. Modification of high saturated fat diet with n-3 polyunsaturated fat improves glucose intolerance and vascular dysfunction. Diabetes Obes Metab 2013; 15(2): 144–52. doi: 10.1111/dom.12004

  13. Ghanbari R, Anwar F, Alkharfy KM, Gilani AH, Saari N. Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-a review. Int J Mol Sci 2012; 13(3): 3291–340. doi: 10.3390/ijms13033291

  14. Schwingshackl L, Hoffmann G. Monounsaturated fatty acids, olive oil and health status: a systematic review and meta-analysis of cohort studies. Lipids Health Dis 2014; 13: 154. doi: 10.1186/1476-511x-13-154

  15. Kris-Etherton PM, Pearson TA, Wan Y, Hargrove RL, Moriarty K, Fishell V, et al. High-monounsaturated fatty acid diets lower both plasma cholesterol and triacylglycerol concentrations. Am J Clin Nutr 1999; 70(6): 1009–15. doi: 10.1093/ajcn/70.6.1009

  16. Gottlieb S. Statement from FDA Commissioner Scott Gottlieb, M.D., on a new qualified health claim for consuming oils with high levels of oleic acid to reduce coronary heart disease risk FDA Statement [19 November 2018]. Available from: https://www.fda.gov/NewsEvents/Newsroom/Press Announcements/UCM626210.htm.

  17. Coates AM, Hill AM, Tan SY. Nuts and cardiovascular disease prevention. Curr Atheroscler Rep 2018; 20(10): 48. doi: 10.1007/s11883-018-0749-3

  18. Zainuddin A, Parkányiová J, Parkányiová L, Pokorný J, Sakurai H. Comparison of oxidative resistance of traditional and high-oleic peanut oils in emulsions. Czech J Food Sci 2018; 22: 136–9. doi: 10.17221/10637-CJFS

  19. Hourihane JO, Bedwani SJ, Dean TP, Warner JO. Randomised, double blind, crossover challenge study of allergenicity of peanut oils in subjects allergic to peanuts. BMJ 1997; 314(7087): 1084–8. doi: 10.1136/bmj.314.7087.1084

  20. Horton JD, Goldstein JL, Brown MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Investig 2002; 109(9): 1125–31. doi: 10.1172/jci15593

  21. Choi YJ, Lee KY, Jung SH, Kim HS, Shim G, Kim MG, et al. Activation of AMPK by berberine induces hepatic lipid accumulation by upregulation of fatty acid translocase CD36 in mice. Toxicol Appl Pharmacol 2017; 316: 74–82. doi: 10.1016/j.taap.2016.12.019

  22. Mihaylova MM, Shaw RJ. The AMPK signalling pathway coordinates cell growth, autophagy and metabolism. Nat Cell Biol 2011; 13(9): 1016–23. doi: 10.1038/ncb2329

  23. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P. Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature 2005; 434(7029): 113–8. doi: 10.1038/nature03354

  24. Chen H, Liu X, Chen H, Cao J, Zhang L, Hu X, et al. Role of SIRT1 and AMPK in mesenchymal stem cells differentiation. Ageing Res Rev 2014; 13: 55–64. doi: 10.1016/j.arr.2013.12.002

  25. Pravenec M, Landa V, Zidek V, Musilova A, Kazdova L, Qi N, et al. Transgenic expression of CD36 in the spontaneously hypertensive rat is associated with amelioration of metabolic disturbances but has no effect on hypertension. Physiol Res 2003; 52(6): 681–8. doi: 10.1152/physiolgenomics.00083.2011

  26. van Schaftingen E, Gerin I. The glucose-6-phosphatase system. Biochem J 2002; 362(Pt 3): 513–32. doi: 10.1042/0264-6021:3620513

  27. Reeves PG, Nielsen FH, Fahey GC, Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993; 123(11): 1939–51. doi: 10.1093/jn/123.11.1939

  28. Mesilati-Stahy R, Mida K, Argov-Argaman N. Size-dependent lipid content of bovine milk fat globule and membrane phospholipids. J Agric Food Chem 2011; 59(13): 7427–35. doi: 10.1021/jf201373j

  29. Eggers LF, Schwudke D. Liquid extraction: Folch. In: Wenk MR, ed. Encyclopedia of lipidomics. Dordrecht, Netherlands: Springer; 2016, pp. 1–6.

  30. Pabinger S, Rödiger S, Kriegner A, Vierlinger K, Weinhäusel A. A survey of tools for the analysis of quantitative PCR (qPCR) data. BDQ 2014; 1(1): 23–33. doi: 10.1016/j.bdq.2014.08.002

  31. Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med 2008; 233(6): 674–88. doi: 10.3181/0711-mr-311

  32. Ferramosca A, Savy V, Zara V. Olive oil increases the hepatic triacylglycerol content in mice by a distinct influence on the synthesis and oxidation of fatty acids. Biosci Biotechnol Biochem 2008; 72(1): 62–9. doi: 10.1271/bbb.70369

  33. Natali F, Siculella L, Salvati S, Gnoni GV. Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells. J Lipid Res 2007; 48(9): 1966–75. doi: 10.1194/jlr.M700051-JLR200

  34. Mattson FH, Grundy SM. Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. J Lipid Res 1985; 26(2): 194–202. PMID: 3989378

  35. Deol P, Evans JR, Dhahbi J, Chellappa K, Han DS, Spindler S, et al. Soybean oil is more obesogenic and diabetogenic than coconut oil and fructose in mouse: potential role for the liver. PLoS One 2015: 10(7): e0132672. doi: 10.1371/journal.pone.0132672

  36. Eberle D, Hegarty B, Bossard P, Ferre P, Foufelle F. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004; 86(11): 839–48. doi: 10.1016/j.biochi.2004.09.018

  37. Zendedel E, Butler AE, Atkin SL, Sahebkar A. Impact of curcumin on sirtuins: a review. J Cell Biochem 2018; 119(12): 10291–300. doi: 10.1002/jcb.27371

  38. Vancura A, Nagar S, Kaur P, Bu P, Bhagwat M, Vancurova I. Reciprocal regulation of AMPK/SNF1 and protein acetylation. Int J Mol Sci 2018; 19(11): E3314. doi: 10.3390/ijms19113314

  39. Luiken JJ, Arumugam Y, Dyck DJ, Bell RC, Pelsers MM, Turcotte LP, et al. Increased rates of fatty acid uptake and plasmalemmal fatty acid transporters in obese Zucker rats. J Biol Chem 2001; 276(44): 40567–73. doi: 10.1074/jbc.M100052200

  40. Aitman TJ, Glazier AM, Wallace CA, Cooper LD, Norsworthy PJ, Wahid FN, et al. Identification of Cd36 (Fat) as an insulin-resistance gene causing defective fatty acid and glucose metabolism in hypertensive rats. Nat Genet 1999; 21(1): 76–83. doi: 10.1038/5013

  41. Wilson CG, Tran JL, Erion DM, Vera NB, Febbraio M, Weiss EJ. Hepatocyte-specific disruption of CD36 attenuates fatty liver and improves insulin sensitivity in HFD-fed mice. Endocrinology 2016; 157(2): 570–85. doi: 10.1210/en.2015-1866

  42. Krammer J, Digel M, Ehehalt F, Stremmel W, Füllekrug J, Ehehalt R. Overexpression of CD36 and acyl-CoA synthetases FATP2, FATP4 and ACSL1 increases fatty acid uptake in human hepatoma cells. Int J Med Sci 2011; 8(7): 599–614. doi: 10.7150/ijms.8.599

  43. Pérez-Jiménez F, Ruano J, Perez-Martinez P, Lopez-Segura F, Lopez-Miranda J. The influence of olive oil on human health: not a question of fat alone. Mol Nutr Food Res 2007; 51(10): 1199–208. doi: 10.1002/mnfr.200600273

  44. Meidan E, Kolesnikov Y, Tirosh O. High fat diets composed of palm stearin and olive oil equally exacerbate liver inflammatory damage and metabolic stress in mice. Mol Nutr Food Res 2018; 62(13): e1700915. doi: 10.1002/mnfr.201700915

How to Cite
Altberg , A., Hovav, R., Chapnik, N., & Madar, Z. (2020). Effect of dietary oils from various sources on carbohydrate and fat metabolism in mice. Food & Nutrition Research, 64. https://doi.org/10.29219/fnr.v64.4287
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